FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2156078257> ?p ?o ?g. }

• W2156078257 abstract "Polyploidy has been recognized for many years as an important hallmark of cancer cells. Polyploid cells can arise through cell fusion, endoreplication and abortive cell cycle. The inner nuclear membrane protein LAP2β plays key roles in nuclear envelope breakdown and reassembly during mitosis, initiation of replication and transcriptional repression. Here we studied the function of LAP2β in the maintenance of cell ploidy state, a role which has not yet been assigned to this protein.By knocking down the expression of LAP2β, using both viral and non-viral RNAi approaches in osteosarcoma derived U2OS cells, we detected enlarged nuclear size, nearly doubling of DNA content and chromosomal duplications, as analyzed by fluorescent in situ hybridization and spectral karyotyping methodologies. Spectral karyotyping analyses revealed that near-hexaploid karyotypes of LAP2β knocked down cells consisted of not only seven duplicated chromosomal markers, as could be anticipated by genome duplication mechanism, but also of four single chromosomal markers. Furthermore, spectral karyotyping analysis revealed that both of two near-triploid U2OS sub-clones contained the seven markers that were duplicated in LAP2β knocked down cells, whereas the four single chromosomal markers were detected only in one of them. Gene expression profiling of LAP2β knocked down cells revealed that up to a third of the genes exhibiting significant changes in their expression are involved in cancer progression.Our results suggest that nuclear fusion mechanism underlies the polyploidization induction upon LAP2β reduced expression. Our study implies on a novel role of LAP2β in the maintenance of cell ploidy status. LAP2β depleted U2OS cells can serve as a model to investigate polyploidy and aneuploidy formation by nuclear fusion mechanism and its involvement in cancerogenesis." @default.
• W2156078257 created "2016-06-24" @default.
• W2156078257 creator A5018573837 @default.
• W2156078257 creator A5029001092 @default.
• W2156078257 creator A5029561913 @default.
• W2156078257 creator A5031725785 @default.
• W2156078257 creator A5039010087 @default.
• W2156078257 creator A5065481823 @default.
• W2156078257 creator A5071994598 @default.
• W2156078257 creator A5082558562 @default.
• W2156078257 date "2014-01-28" @default.
• W2156078257 modified "2023-10-17" @default.
• W2156078257 title "Induction of polyploidy by nuclear fusion mechanism upon decreased expression of the nuclear envelope protein LAP2β in the human osteosarcoma cell line U2OS" @default.
• W2156078257 cites W1538500488 @default.
• W2156078257 cites W1607487366 @default.
• W2156078257 cites W1777304034 @default.
• W2156078257 cites W1845088912 @default.
• W2156078257 cites W1965168929 @default.
• W2156078257 cites W1969081908 @default.
• W2156078257 cites W1993222258 @default.
• W2156078257 cites W2004629460 @default.
• W2156078257 cites W2008627600 @default.
• W2156078257 cites W2012471677 @default.
• W2156078257 cites W2014339999 @default.
• W2156078257 cites W2014402428 @default.
• W2156078257 cites W2015452754 @default.
• W2156078257 cites W2022989793 @default.
• W2156078257 cites W2023806461 @default.
• W2156078257 cites W2036970799 @default.
• W2156078257 cites W2038011290 @default.
• W2156078257 cites W2043485308 @default.
• W2156078257 cites W2045802318 @default.
• W2156078257 cites W2052833562 @default.
• W2156078257 cites W2062862969 @default.
• W2156078257 cites W2067424975 @default.
• W2156078257 cites W2069084191 @default.
• W2156078257 cites W2071059040 @default.
• W2156078257 cites W2080125465 @default.
• W2156078257 cites W2087067430 @default.
• W2156078257 cites W2094615509 @default.
• W2156078257 cites W2098878044 @default.
• W2156078257 cites W2103330925 @default.
• W2156078257 cites W2107190139 @default.
• W2156078257 cites W2121212012 @default.
• W2156078257 cites W2122382992 @default.
• W2156078257 cites W2122683221 @default.
• W2156078257 cites W2140998213 @default.
• W2156078257 cites W2145033500 @default.
• W2156078257 cites W2155884272 @default.
• W2156078257 cites W2157993533 @default.
• W2156078257 cites W2158217645 @default.
• W2156078257 cites W4211059758 @default.
• W2156078257 cites W4243158166 @default.
• W2156078257 cites W96788818 @default.
• W2156078257 doi "https://doi.org/10.1186/1755-8166-7-9" @default.
• W2156078257 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3926685" @default.
• W2156078257 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24472424" @default.
• W2156078257 hasPublicationYear "2014" @default.
• W2156078257 type Work @default.
• W2156078257 sameAs 2156078257 @default.
• W2156078257 citedByCount "8" @default.
• W2156078257 countsByYear W21560782572015 @default.
• W2156078257 countsByYear W21560782572016 @default.
• W2156078257 countsByYear W21560782572017 @default.
• W2156078257 countsByYear W21560782572021 @default.
• W2156078257 crossrefType "journal-article" @default.
• W2156078257 hasAuthorship W2156078257A5018573837 @default.
• W2156078257 hasAuthorship W2156078257A5029001092 @default.
• W2156078257 hasAuthorship W2156078257A5029561913 @default.
• W2156078257 hasAuthorship W2156078257A5031725785 @default.
• W2156078257 hasAuthorship W2156078257A5039010087 @default.
• W2156078257 hasAuthorship W2156078257A5065481823 @default.
• W2156078257 hasAuthorship W2156078257A5071994598 @default.
• W2156078257 hasAuthorship W2156078257A5082558562 @default.
• W2156078257 hasBestOaLocation W21560782571 @default.
• W2156078257 hasConcept C145226528 @default.
• W2156078257 hasConcept C1491633281 @default.
• W2156078257 hasConcept C153911025 @default.
• W2156078257 hasConcept C29537977 @default.
• W2156078257 hasConcept C54355233 @default.
• W2156078257 hasConcept C86803240 @default.
• W2156078257 hasConcept C93304396 @default.
• W2156078257 hasConcept C95444343 @default.
• W2156078257 hasConceptScore W2156078257C145226528 @default.
• W2156078257 hasConceptScore W2156078257C1491633281 @default.
• W2156078257 hasConceptScore W2156078257C153911025 @default.
• W2156078257 hasConceptScore W2156078257C29537977 @default.
• W2156078257 hasConceptScore W2156078257C54355233 @default.
• W2156078257 hasConceptScore W2156078257C86803240 @default.
• W2156078257 hasConceptScore W2156078257C93304396 @default.
• W2156078257 hasConceptScore W2156078257C95444343 @default.
• W2156078257 hasIssue "1" @default.
• W2156078257 hasLocation W21560782571 @default.
• W2156078257 hasLocation W21560782572 @default.
• W2156078257 hasLocation W21560782573 @default.
• W2156078257 hasLocation W21560782574 @default.
• W2156078257 hasOpenAccess W2156078257 @default.
• W2156078257 hasPrimaryLocation W21560782571 @default.
• W2156078257 hasRelatedWork W1969408338 @default.
• W2156078257 hasRelatedWork W2013318939 @default.

• next