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- W2156156731 abstract "Measurement of unconjugated cortisol in the urine affords a reliable index of the biologically active fraction of circulating cortisol useful in the diagnosis of Cushing's syndrome. A simple and accurate technique using glass fiber chromatography was developed to determine unconjugated urinary cortisol. One hundred ml of urine was extracted with dichloromethane without previous hydrolysis, applied to glass fiber sheets and run in 1 or 2 different chromatographic systems for 20 min. Quantitation was performed by the Porter-Silber reaction, and values were corrected for losses by means of added tracer amounts of ortisol-4-C14. Mean recovery was 80.5%, and replicate determinations had an average deviation from the mean of 5.5%. Thirty-eight normal subjects studied with this technique had a mean value of urinary unconjugated cortisol of 71.4 μg/day, with arange of 0–181. Nine patients with Cushing's syndrome had values of 297 to 3,605 μg/day. In thyrotoxic and obese patients with elevated urinary 17-hydroxyco..." @default.
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- W2156156731 date "1963-08-01" @default.
- W2156156731 modified "2023-09-27" @default.
- W2156156731 title "Determination of Urinary Unconjugated Cortisol by Glass Fiber Chromatography in the Diagnosis of Cushing's Syndrome1" @default.
- W2156156731 doi "https://doi.org/10.1210/jcem-23-8-820" @default.
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