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• W2156202232 endingPage "668" @default.
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• W2156202232 abstract "MADS-box transcriptional regulators play important roles during plant development. Based on phylogenetic reconstruction, the AP1/SEP/AGL6 superclade of floral MADS-box genes underwent one or two duplication events in the common ancestor of the core eudicots. However, the functional evolution of the AP1/SEP/AGL6 superclade in basal eudicots remains uncharacterized. Epimedium sagittatum is a basal eudicot species valued for its medicinal properties and showing unique floral morphology. In this study, structural and functional variation of FUL-like (AP1 subfamily), SEP-like and AGL6-like genes in this species was investigated to further our understanding of flower evolution in angiosperms. Detailed investigations into the microsynteny and evolutionary history of the floral A and E class MADS-box genes in eudicots were undertaken and used to trace their genomic rearrangements.One AP1-like gene, two SEP-like genes and one AGL6-like gene were cloned from E. sagittatum. Their expression patterns were examined using quantitative RT-PCR in different vegetative and reproductive organs at two developmental stages. Yeast two-hybrid assays were carried out among AP1/SEP/AGL6 superclade, AP3/PI and AGAMOUS subfamily members for elucidation of dimerization patterns. In addition, possible formation of a ternary complex involving B class proteins with the A class protein EsFUL-like, the E class SEP-like protein EsAGL2-1 or the AGL6-class protein EsAGL6 were detected using yeast three-hybrid assays. Transgenic Arabidopsis or tobacco plants expressing EsFUL-like, EsAGL2-1 and EsAGL6-like under the cauliflower mosaic virus (CaMV) 35S promoter were generated and analysed. Genomic studies of AP1 syntenic regions in arabidopsis, columbine, strawberry, papaya, peach, grapevine and tomato were conducted for microsyntenic analyses.Sequence and phylogenetic analyses showed that EsFUL-like is a member of the AP1 (A class) subfamily, EsAGL2-1 and EsAGL2-2 belong to the SEP-like (E class) subfamily, and EsAGL6-like belongs to the AGL6 (AGL6 class) subfamily. Quantitative RT-PCR analyses revealed that the transcripts of the four genes are absent, or minimal, in vegetative tissues and are most highly expressed in floral organs. Yeast two-hybrid results revealed that of the eight MADS-box proteins tested, only EsAGL6-like, EsAGL2-1 and EsAGL2 were able to form strong homo- and heterodimers, with EsAGL6-like and EsAGL2-1 showing similar interaction patterns. Yeast three-hybrid analysis revealed that EsFUL1-like, EsAGL6-like and EsAGL2-1 (representing the three major lineages of the Epimedium AGL/SEP/ALG6 superclade) could act as bridging proteins in ternary complexes with both EsAP3-2 (B class) and EsPI (B class), which do not heterodimerize themselves. Syntenic analyses of sequenced basal eudicots, rosids and asterids showed that most AP1-like and SEP-like genes have been tightly associated as neighbours since the origin of basal eudicots. Ectopic expression of EsFUL-like in arabidopsis caused early flowering through endogenous high-level expression of AP1 and formation of secondary flowers between the first and second whorls. Tobacco plants with ectopic expression of EsAGL2-1 showed shortened pistils and styles, as well as axillary and extra petals in the initial flower.This study provides a description of EsFUL-like, EsAGL2-1, EsAGL2-2 and EsAGL6-like function divergence and conservation in comparison with a selection of model core eudicots. The study also highlights how organization in genomic segments containing A and E class genes in sequenced model species has resulted in similar topologies of AP1 and SEP-like gene trees." @default.
• W2156202232 created "2016-06-24" @default.
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• W2156202232 date "2014-02-13" @default.
• W2156202232 modified "2023-10-18" @default.
• W2156202232 title "Functional and evolutionary analysis of the AP1/SEP/AGL6 superclade of MADS-box genes in the basal eudicot Epimedium sagittatum" @default.
• W2156202232 cites W1612066627 @default.
• W2156202232 cites W1951967101 @default.
• W2156202232 cites W1965330337 @default.
• W2156202232 cites W1985241409 @default.
• W2156202232 cites W1987300180 @default.
• W2156202232 cites W1994145712 @default.
• W2156202232 cites W1995191951 @default.
• W2156202232 cites W1995759424 @default.
• W2156202232 cites W2001354000 @default.
• W2156202232 cites W2001374342 @default.
• W2156202232 cites W2003198924 @default.
• W2156202232 cites W2014832104 @default.
• W2156202232 cites W2025083770 @default.
• W2156202232 cites W2030126704 @default.
• W2156202232 cites W2030948581 @default.
• W2156202232 cites W2032117740 @default.
• W2156202232 cites W2041122198 @default.
• W2156202232 cites W2043184835 @default.
• W2156202232 cites W2043359041 @default.
• W2156202232 cites W2045219431 @default.
• W2156202232 cites W2045429591 @default.
• W2156202232 cites W2046944468 @default.
• W2156202232 cites W2055952401 @default.
• W2156202232 cites W2056871145 @default.
• W2156202232 cites W2058151615 @default.
• W2156202232 cites W2059291619 @default.
• W2156202232 cites W2064395884 @default.
• W2156202232 cites W2066812913 @default.
• W2156202232 cites W2066841887 @default.
• W2156202232 cites W2071636376 @default.
• W2156202232 cites W2072605733 @default.
• W2156202232 cites W2088994407 @default.
• W2156202232 cites W2089283973 @default.
• W2156202232 cites W2094617362 @default.
• W2156202232 cites W2095621415 @default.
• W2156202232 cites W2100144868 @default.
• W2156202232 cites W2104827882 @default.
• W2156202232 cites W2106214237 @default.
• W2156202232 cites W2106676265 @default.
• W2156202232 cites W2110840887 @default.
• W2156202232 cites W2110904645 @default.
• W2156202232 cites W2111900469 @default.
• W2156202232 cites W2114696020 @default.
• W2156202232 cites W2115185640 @default.
• W2156202232 cites W2115880496 @default.
• W2156202232 cites W2116122104 @default.
• W2156202232 cites W2117175296 @default.
• W2156202232 cites W2117989671 @default.
• W2156202232 cites W2121265967 @default.
• W2156202232 cites W2124548890 @default.
• W2156202232 cites W2124819931 @default.
• W2156202232 cites W2126446831 @default.
• W2156202232 cites W2130327038 @default.
• W2156202232 cites W2132130295 @default.
• W2156202232 cites W2133191324 @default.
• W2156202232 cites W2133757548 @default.
• W2156202232 cites W2135604818 @default.
• W2156202232 cites W2137167825 @default.
• W2156202232 cites W2142124646 @default.
• W2156202232 cites W2146254013 @default.
• W2156202232 cites W2146590860 @default.
• W2156202232 cites W2148037134 @default.
• W2156202232 cites W2154892216 @default.
• W2156202232 cites W2156527221 @default.
• W2156202232 cites W2156594781 @default.
• W2156202232 cites W2156923490 @default.
• W2156202232 cites W2157455385 @default.
• W2156202232 cites W2157700221 @default.
• W2156202232 cites W2157900320 @default.
• W2156202232 cites W2162068332 @default.
• W2156202232 cites W2162697712 @default.
• W2156202232 cites W2167932128 @default.
• W2156202232 cites W2170161119 @default.
• W2156202232 cites W2170869586 @default.
• W2156202232 cites W4254275792 @default.
• W2156202232 doi "https://doi.org/10.1093/aob/mct301" @default.
• W2156202232 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3936592" @default.
• W2156202232 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24532606" @default.
• W2156202232 hasPublicationYear "2014" @default.
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