FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2156225399> ?p ?o ?g. }

• W2156225399 endingPage "3852" @default.
• W2156225399 startingPage "3842" @default.
• W2156225399 abstract "ABSTRACT Artemisinin-based combination therapies (ACTs) are highly effective for the treatment of Plasmodium falciparum malaria, yet their sustained efficacy is threatened by the potential spread of parasite resistance. Recent studies have provided evidence that artemisinins can inhibit the function of PfATP6, the P. falciparum ortholog of the ER calcium pump SERCA, when expressed in Xenopus laevis oocytes. Inhibition was significantly reduced in an L263E variant, which introduced the mammalian residue into a putative drug-binding pocket. To test the hypothesis that this single mutation could decrease P. falciparum susceptibility to artemisinins, we implemented an allelic-exchange strategy to replace the wild-type pfatp6 allele by a variant allele encoding L263E. Transfected P. falciparum clones were screened by PCR analysis for disruption of the endogenous locus and introduction of the mutant L263E allele under the transcriptional control of a calmodulin promoter. Expression of the mutant allele was demonstrated by reverse transcriptase (RT) PCR and verified by sequence analysis. Parasite clones expressing wild-type or L263E variant PfATP6 showed no significant difference in 50% inhibitory concentrations (IC 50 s) for artemisinin or its derivatives dihydroartemisinin and artesunate. Nonetheless, hierarchical clustering analysis revealed a trend toward reduced susceptibility that neared significance (artemisinin, P ≈ 0.1; dihydroartemisinin, P = 0.053 and P = 0.085; and artesunate, P = 0.082 and P = 0.162 for the D10 and 7G8 lines, respectively). Notable differences in the distribution of normalized IC 50 s provided evidence of decreased responsiveness to artemisinin and dihydroartemisinin ( P = 0.02 for the D10 and 7G8 lines), but not to artesunate in parasites expressing mutant PfATP6." @default.
• W2156225399 created "2016-06-24" @default.
• W2156225399 creator A5031846220 @default.
• W2156225399 creator A5066476364 @default.
• W2156225399 creator A5071314941 @default.
• W2156225399 creator A5088222987 @default.
• W2156225399 creator A5091489538 @default.
• W2156225399 date "2010-09-01" @default.
• W2156225399 modified "2023-10-03" @default.
• W2156225399 title "Investigations into the Role of the <i>Plasmodium falciparum</i> SERCA (PfATP6) L263E Mutation in Artemisinin Action and Resistance" @default.
• W2156225399 cites W1827803632 @default.
• W2156225399 cites W1865107143 @default.
• W2156225399 cites W1963728755 @default.
• W2156225399 cites W1967127038 @default.
• W2156225399 cites W1971614704 @default.
• W2156225399 cites W1972171883 @default.
• W2156225399 cites W1972177048 @default.
• W2156225399 cites W1972178220 @default.
• W2156225399 cites W1973696943 @default.
• W2156225399 cites W1974832244 @default.
• W2156225399 cites W1981089872 @default.
• W2156225399 cites W1983733343 @default.
• W2156225399 cites W1989434001 @default.
• W2156225399 cites W1996418116 @default.
• W2156225399 cites W2000312340 @default.
• W2156225399 cites W2001276105 @default.
• W2156225399 cites W2002565384 @default.
• W2156225399 cites W2008678761 @default.
• W2156225399 cites W2022012565 @default.
• W2156225399 cites W2033721665 @default.
• W2156225399 cites W2035758606 @default.
• W2156225399 cites W2037350416 @default.
• W2156225399 cites W2040713555 @default.
• W2156225399 cites W2042237497 @default.
• W2156225399 cites W2048271518 @default.
• W2156225399 cites W2051905139 @default.
• W2156225399 cites W2052295036 @default.
• W2156225399 cites W2060322845 @default.
• W2156225399 cites W2076029682 @default.
• W2156225399 cites W2076870550 @default.
• W2156225399 cites W2083001268 @default.
• W2156225399 cites W2084313021 @default.
• W2156225399 cites W2103460690 @default.
• W2156225399 cites W2108002481 @default.
• W2156225399 cites W2108523194 @default.
• W2156225399 cites W2112047656 @default.
• W2156225399 cites W2114705206 @default.
• W2156225399 cites W2119815654 @default.
• W2156225399 cites W2125397010 @default.
• W2156225399 cites W2130941208 @default.
• W2156225399 cites W2137529385 @default.
• W2156225399 cites W2138754677 @default.
• W2156225399 cites W2139878820 @default.
• W2156225399 cites W2150085419 @default.
• W2156225399 cites W2153331570 @default.
• W2156225399 cites W2155471509 @default.
• W2156225399 cites W2160689391 @default.
• W2156225399 cites W2161311583 @default.
• W2156225399 cites W2163774316 @default.
• W2156225399 cites W2164365774 @default.
• W2156225399 cites W2167912850 @default.
• W2156225399 cites W2168918061 @default.
• W2156225399 cites W2344075384 @default.
• W2156225399 cites W2396712276 @default.
• W2156225399 cites W4211035227 @default.
• W2156225399 cites W4244358260 @default.
• W2156225399 doi "https://doi.org/10.1128/aac.00121-10" @default.
• W2156225399 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2935017" @default.
• W2156225399 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20566762" @default.
• W2156225399 hasPublicationYear "2010" @default.
• W2156225399 type Work @default.
• W2156225399 sameAs 2156225399 @default.
• W2156225399 citedByCount "54" @default.
• W2156225399 countsByYear W21562253992012 @default.
• W2156225399 countsByYear W21562253992013 @default.
• W2156225399 countsByYear W21562253992014 @default.
• W2156225399 countsByYear W21562253992015 @default.
• W2156225399 countsByYear W21562253992016 @default.
• W2156225399 countsByYear W21562253992017 @default.
• W2156225399 countsByYear W21562253992018 @default.
• W2156225399 countsByYear W21562253992019 @default.
• W2156225399 countsByYear W21562253992020 @default.
• W2156225399 countsByYear W21562253992021 @default.
• W2156225399 countsByYear W21562253992022 @default.
• W2156225399 crossrefType "journal-article" @default.
• W2156225399 hasAuthorship W2156225399A5031846220 @default.
• W2156225399 hasAuthorship W2156225399A5066476364 @default.
• W2156225399 hasAuthorship W2156225399A5071314941 @default.
• W2156225399 hasAuthorship W2156225399A5088222987 @default.
• W2156225399 hasAuthorship W2156225399A5091489538 @default.
• W2156225399 hasBestOaLocation W21562253992 @default.
• W2156225399 hasConcept C104317684 @default.
• W2156225399 hasConcept C143065580 @default.
• W2156225399 hasConcept C153911025 @default.
• W2156225399 hasConcept C180754005 @default.
• W2156225399 hasConcept C203014093 @default.
• W2156225399 hasConcept C2776120307 @default.
• W2156225399 hasConcept C2778048844 @default.

• next