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• W2156367056 abstract "There is an increasing demand for novel high-throughput screening (HTS) technologies in the pharmaceutical and biotechnological industries. The robotic sample-handling techniques currently used in these industries, although fast, are still limited to operating in multiwell plates with the sample volumes per reaction in the microliter regime. Digital microfluidics offers an alternative for reduction in sample volume consumption for HTS but lacks a reliable technique for transporting a large number of samples to the microfluidic device. In this report, we develop a technique for serial delivery of sample arrays to a microfluidic device from multiwell plates, through a single sample inlet. Under this approach, a serial array of sample plugs, separated by an immiscible carrier fluid, is loaded into a capillary and delivered to a microfluidic device. Similar approaches have been attempted in the past, however, either with a slower sample loading device such as a syringe pump or vacuum-based sample loading with limited driving pressure. We demonstrated the application of our positive-pressure–based serial sample loading (SSL) system to load a series of sample plugs into a capillary. The adaptability of the SSL system to generate sample plugs with a variety of volumes in a predictable manner was also demonstrated." @default.
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• W2156367056 date "2012-10-01" @default.
• W2156367056 modified "2023-10-14" @default.
• W2156367056 title "A Serial Sample Loading System: Interfacing Multiwell Plates with Microfluidic Devices" @default.
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• W2156367056 doi "https://doi.org/10.1177/2211068212455169" @default.
• W2156367056 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3696387" @default.
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