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• W2156408641 abstract "The two major epigenetic modifications of cytosines, 5-methylcytosine (5-mC) and 5-hydroxymethylcytosine (5-hmC), coexist with each other in a range of mammalian cell populations. Increasing evidence points to important roles of 5-hmC in demethylation of 5-mC and epigenomic regulation in development. Recently developed experimental methods allow direct single-base profiling of either 5-hmC or 5-mC. Meaningful analyses seem to require combining these experiments with bisulfite sequencing, but doing so naively produces inconsistent estimates of 5-mC or 5-hmC levels.We present a method to jointly model read counts from bisulfite sequencing, oxidative bisulfite sequencing and Tet-Assisted Bisulfite sequencing, providing simultaneous estimates of 5-hmC and 5-mC levels that are consistent across experiment types.http://smithlab.usc.edu/software/mlml" @default.
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• W2156408641 date "2013-08-21" @default.
• W2156408641 modified "2023-10-10" @default.
• W2156408641 title "MLML: consistent simultaneous estimates of DNA methylation and hydroxymethylation" @default.
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• W2156408641 doi "https://doi.org/10.1093/bioinformatics/btt459" @default.
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