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- W2156486173 abstract "Publisher Summary The specificity of the solid-phase minisequencing method originates from the fidelity of the nucleotide incorporation catalyzed by the deoxyribonucleic acid (DNA) polymerase, whereas the primer annealing reaction is performed at nonstringent conditions. This chapter describes the application of a multiplex, solid-phase minisequencing method for simultaneous detection of four polymorphisms (*3, *4, *5, *6) in CYP2D6 and *2 in 2C19 genes using fluorescently labeled ddNTPs. It should be noted that the CYP2D6*5 allele is a complete gene deletion. It also describes the principle of the multiplex minisequencing method and design and synthesis of primers. Also, a specific protocol for typing four polymorphisms in CYP2D6 and 2C19 genes is provided. All reagents and equipment required for the fluorescent, multiplex minisequencing method described here are available from common suppliers of molecular biological reagents. Therefore, the method is easy to set up both in the research laboratory and for routine analysis of previously known sequence variants. The procedure involves simple and robust treatment procedures. In particular, the manifold format of the minisequencing step, facilities handling procedures and interpretation of the results are simple. Furthermore, the method is highly flexible and mutant alleles may easily be added or subtracted to a given procedure as desired." @default.
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- W2156486173 date "1999-01-01" @default.
- W2156486173 modified "2023-10-16" @default.
- W2156486173 title "A fluorescent, multiplex solid-phase minisequencing method for genotyping cytochrome P450 genes" @default.
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- W2156486173 doi "https://doi.org/10.1016/b978-012372185-3/50035-3" @default.
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