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• W2156558869 abstract "At least eight species of Cryptosporidium can cause human infection and disease. A real-time PCR (qPCR) assay based on the 18S rRNA gene and utilizing a Scorpion probe was developed to detect all human-pathogenic Cryptosporidium without the usual need for nested amplification. Sensitivity of detection in stool samples was highest using a glass bead-based DNA extraction method (under 10 3 oocysts per stool sample). The assay was validated against 123 human stool specimens from Bangladesh and Tanzania, exhibited a sensitivity and specificity of >91 % versus microscopy, and detected an additional eight microscopy-negative infections. Cryptosporidium parvum -specific and Cryptosporidium meleagridis -specific Scorpion qPCR assays that provided 100 % accurate speciation compared with Vsp I RFLP analysis and sequencing were developed subsequently. These Scorpion probe qPCR assays are simpler to perform than existing nested PCR and RFLP methods for diagnosis and epidemiological investigation of cryptosporidiosis." @default.
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• W2156558869 date "2006-09-01" @default.
• W2156558869 modified "2023-09-27" @default.
• W2156558869 title "Real-time PCR detection and speciation of Cryptosporidium infection using Scorpion probes" @default.
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• W2156558869 doi "https://doi.org/10.1099/jmm.0.46678-0" @default.
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