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- W2156559600 abstract "Summary Selection-marker genes are routinely used in plant genetic transformation protocols to ensure the survival of transformed cells by limiting the regeneration of non-transgenic cells after transformation. In order to find alternatives to the use of antibiotics as selection agents we focused on so-called positive selection systems. Four alternative systems were investigated for their potential as selection agents in sugarcane genetic transformation. The systems included i) galactose, ii) 2-deoxyglucose iii) NaCl and iv) gluconic acid as selective agents in combination with the galactose-�-phoshate uridyl-transferase (galT), 2-deoxyglucose-6-phosphate-phosphatase (2Dog6PP), cytosolic betaine aldehyde dehydrogenase (BADH), and gluconokinase selection genes, respectively. Control sugarcane callus sensitivity towards these selection agents were determined and resulted in gluconic acid being eliminated as a possible selection agent. Transgenic calli were produced for the remaining three selection systems and results monitored. Galactose and 2-deoxyglucose showed the most promise as alternative selection agents in the production of transgenic sugarcane callus." @default.
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- W2156559600 date "2009-01-01" @default.
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- W2156559600 title "Establishment of alternative selection systems for transgenic sugarcane callus." @default.
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