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• W2156585249 abstract "ABSTRACT We determined the nucleotide sequences (329 bp) of the rpoB DNAs from 22 reference strains of Borrelia . No insertions or deletions were observed. Deduced amino acid sequences of amplified rpoB DNA comprised 109 amino acid residues (N 450 to M 558 [ Escherichia coli numbering]). All amino acid sequences were identical with the exception of those of Borrelia lusitaniae PotiB2 (T 461 →A) and B. bissettii DN127 (I 498 →V). Each species of B. burgdorferi sensu lato was differentiated as a distinct entity in the phylogenetic tree constructed by a neighbor-joining method. B. burgdorferi sensu lato could be distinguished from B. turicatae and B. hermsii , which are associated with relapsing fever. Seventeen Korean isolates could be identified by PCR-linked direct sequencing and restriction analysis of the rpoB DNA. These results suggest that rpoB DNA is useful for identification and characterization of Borrelia . In addition, we developed the rapid species identification method using the species-specific primer sets based on rpoB gene sequences." @default.
• W2156585249 created "2016-06-24" @default.
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• W2156585249 date "2000-07-01" @default.
• W2156585249 modified "2023-10-11" @default.
• W2156585249 title "Differentiation of <i>Borrelia burgdorferi</i> Sensu Lato on the Basis of RNA Polymerase Gene ( <i>rpoB</i> ) Sequences" @default.
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• W2156585249 doi "https://doi.org/10.1128/jcm.38.7.2557-2562.2000" @default.
• W2156585249 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/86967" @default.
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