Matches in SemOpenAlex for { <https://semopenalex.org/work/W2156670210> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2156670210 endingPage "194" @default.
- W2156670210 startingPage "185" @default.
- W2156670210 abstract "US FDA and EMEA guidance recommend that the preferred in vitro model for cytochrome P450 induction testing is human hepatocytes coupled with acceptable inducers as controls. However, there are surprisingly few published studies characterizing this model system for dose and time-dependence response to model inducing compounds. The concentration- dependent response and time-course for the induction of CYP1A2, CYP2B6 and CYP3A4 by inducing agents β-naphthoflavone, phenobarbital and rifampicin, respectively were examined in two or more donors using multiple endpoints (mRNA, enzyme activity and Western blot analysis). Depending on the endpoint, exposure time for maximal response of CYP induction potential for the three enzymes ranged from 24 to 72 hours. Of the concentrations of BNF, PB and RIF tested, those which gave the maximal response were found to be 33 μM, > 2 mM and 10 μM, respectively. Keywords: Cytochrome P450, induction, human hepatocytes, concentration and time-dependence, Naphthoflavone, Phenobarbital, Rifampicin, hepatocytes, time-dependence response, CYP1A2, CYP2B6, CYP3A4, multiple end-points, concentration, time-dependence, drug-metabolizing enzymes, enzyme activity, Hepatocyte Culture, Hepatocytes plated, Induction Response, CYP Activity, Prototypical Inducers, Reverse Tran-scriptase Polymerase Chain Reaction (RT-PCR), Western Blot Analysis, Cell lysate, lysate protein, immunoblot response, chemiluminescence, fold-induction response, Time-Dependent Induction, Inhibitory Effect, carcinogenic polycyclic aromatic hydro-carbon, Aryl hydrocarbon receptor, Constitutive androstane receptor, Pregnane X receptor" @default.
- W2156670210 created "2016-06-24" @default.
- W2156670210 creator A5004642216 @default.
- W2156670210 creator A5005921835 @default.
- W2156670210 creator A5020868363 @default.
- W2156670210 creator A5030715821 @default.
- W2156670210 creator A5088332392 @default.
- W2156670210 date "2010-12-01" @default.
- W2156670210 modified "2023-10-12" @default.
- W2156670210 title "A Multi-Endpoint Evaluation of Cytochrome P450 1A2, 2B6 and 3A4 Induction Response in Human Hepatocyte Cultures After Treatment with β - Naphthoflavone, Phenobarbital and Rifampicin" @default.
- W2156670210 doi "https://doi.org/10.2174/187231210792928224" @default.
- W2156670210 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20583967" @default.
- W2156670210 hasPublicationYear "2010" @default.
- W2156670210 type Work @default.
- W2156670210 sameAs 2156670210 @default.
- W2156670210 citedByCount "27" @default.
- W2156670210 countsByYear W21566702102012 @default.
- W2156670210 countsByYear W21566702102013 @default.
- W2156670210 countsByYear W21566702102014 @default.
- W2156670210 countsByYear W21566702102015 @default.
- W2156670210 countsByYear W21566702102018 @default.
- W2156670210 countsByYear W21566702102020 @default.
- W2156670210 countsByYear W21566702102021 @default.
- W2156670210 countsByYear W21566702102023 @default.
- W2156670210 crossrefType "journal-article" @default.
- W2156670210 hasAuthorship W2156670210A5004642216 @default.
- W2156670210 hasAuthorship W2156670210A5005921835 @default.
- W2156670210 hasAuthorship W2156670210A5020868363 @default.
- W2156670210 hasAuthorship W2156670210A5030715821 @default.
- W2156670210 hasAuthorship W2156670210A5088332392 @default.
- W2156670210 hasConcept C104317684 @default.
- W2156670210 hasConcept C109650736 @default.
- W2156670210 hasConcept C132040763 @default.
- W2156670210 hasConcept C132154277 @default.
- W2156670210 hasConcept C153911025 @default.
- W2156670210 hasConcept C181199279 @default.
- W2156670210 hasConcept C185592680 @default.
- W2156670210 hasConcept C193264327 @default.
- W2156670210 hasConcept C202751555 @default.
- W2156670210 hasConcept C2225953 @default.
- W2156670210 hasConcept C2776200302 @default.
- W2156670210 hasConcept C2778793413 @default.
- W2156670210 hasConcept C33594762 @default.
- W2156670210 hasConcept C50605568 @default.
- W2156670210 hasConcept C526171541 @default.
- W2156670210 hasConcept C55493867 @default.
- W2156670210 hasConcept C63932345 @default.
- W2156670210 hasConcept C86339819 @default.
- W2156670210 hasConcept C86745063 @default.
- W2156670210 hasConcept C86803240 @default.
- W2156670210 hasConcept C98274493 @default.
- W2156670210 hasConceptScore W2156670210C104317684 @default.
- W2156670210 hasConceptScore W2156670210C109650736 @default.
- W2156670210 hasConceptScore W2156670210C132040763 @default.
- W2156670210 hasConceptScore W2156670210C132154277 @default.
- W2156670210 hasConceptScore W2156670210C153911025 @default.
- W2156670210 hasConceptScore W2156670210C181199279 @default.
- W2156670210 hasConceptScore W2156670210C185592680 @default.
- W2156670210 hasConceptScore W2156670210C193264327 @default.
- W2156670210 hasConceptScore W2156670210C202751555 @default.
- W2156670210 hasConceptScore W2156670210C2225953 @default.
- W2156670210 hasConceptScore W2156670210C2776200302 @default.
- W2156670210 hasConceptScore W2156670210C2778793413 @default.
- W2156670210 hasConceptScore W2156670210C33594762 @default.
- W2156670210 hasConceptScore W2156670210C50605568 @default.
- W2156670210 hasConceptScore W2156670210C526171541 @default.
- W2156670210 hasConceptScore W2156670210C55493867 @default.
- W2156670210 hasConceptScore W2156670210C63932345 @default.
- W2156670210 hasConceptScore W2156670210C86339819 @default.
- W2156670210 hasConceptScore W2156670210C86745063 @default.
- W2156670210 hasConceptScore W2156670210C86803240 @default.
- W2156670210 hasConceptScore W2156670210C98274493 @default.
- W2156670210 hasIssue "4" @default.
- W2156670210 hasLocation W21566702101 @default.
- W2156670210 hasLocation W21566702102 @default.
- W2156670210 hasOpenAccess W2156670210 @default.
- W2156670210 hasPrimaryLocation W21566702101 @default.
- W2156670210 hasRelatedWork W1973422270 @default.
- W2156670210 hasRelatedWork W2008656337 @default.
- W2156670210 hasRelatedWork W2035465414 @default.
- W2156670210 hasRelatedWork W2128266687 @default.
- W2156670210 hasRelatedWork W2138133935 @default.
- W2156670210 hasRelatedWork W2144038328 @default.
- W2156670210 hasRelatedWork W2160195645 @default.
- W2156670210 hasRelatedWork W2168103670 @default.
- W2156670210 hasRelatedWork W2728667519 @default.
- W2156670210 hasRelatedWork W3159825576 @default.
- W2156670210 hasVolume "4" @default.
- W2156670210 isParatext "false" @default.
- W2156670210 isRetracted "false" @default.
- W2156670210 magId "2156670210" @default.
- W2156670210 workType "article" @default.