FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2156815577> ?p ?o ?g. }

• W2156815577 abstract "ENGLISH ABSTRACT: Antimicrobial resistance is one of the major problems faced by the medical industry today. The ability of bacteria to rapidly acquire resistance against antibiotics and the over prescription and inappropriate use of antibiotics further exacerbate this crisis. Few new antimicrobials are, however, making it through the drug discovery pipeline. The search and development of novel and effective antimicrobials is therefore of the utmost importance.Lantibiotics are ribosomally synthesized cationic antimicrobial peptides with extensive post-translational modifications. They are active against a wide range of Gram-positive bacteria, including antibiotic-resistant strains. They are characterized by the presence of lanthionine and methyllanthionine rings and have been suggested as alternatives or for use in conjunction with antibiotics against resistant pathogens. Staphylococcus aureus is the most common bacteria isolated from skin and soft tissue infections (SSTIs). Strains of S. aureus have emerged with resistance against antibiotics with the most common being methicillin-resistant S. aureus (MRSA). Several lantibiotics are active against MRSA in vivo and have even shown superior activity to traditional antibiotics. Lantibiotics therefore show much promise for the treatment of SSTIs caused by resistant- and non-resistant S. aureus.In this study the bacterially diverse soil of the Fynbos in the Western Cape was screened for novel antimicrobials. Two antimicrobial producing Bacillus strains were isolated, Bacillus clausii AD1 and Bacillus amyloliquefaciens AD2. Both of these strains produce lantibiotics with B. clausii AD1 producing a known lantibiotic, clausin. B. amyloliquefaciens AD2 produces a novel two-component lantibiotic which was designated amyloliquecidin. The lantibiotic operon of amyloliquecidin was sequenced and annotated. All the genes required for successful production of amyloliquecidin are present in the operon. Amyloliquecidin was characterized in vitro and along with clausin is active against clinical strains of S. aureus (including MRSA), Enterococcus spp., Listeria spp. and beta-haemolytic streptococci. Amyloliquecidin has remarkable stability at physiological pH compared to nisin and clausin. A comparative in vivo murine infection model was used to evaluate the effectiveness of amyloliquecidin, nisin, clausin and Bactroban (commercial S. aureus topical treatment) in treating wound infections caused by S. aureus. All the lantibiotics proved to be just as effective as the Bactroban treatment. Furthermore, the tested lantibiotics did not have a negative influence on the wound closure rates of infected and non-infected wounds. Bactroban had a negative effect on wound healing compared to the lantibiotics.To our knowledge amyloliquecidin is the third two-component lantibiotic isolated from Bacillus. This study represents the first to test the effectiveness of amyloliquecidin in vivo and is one of a handful to test lantibiotics as topical treatments.%%%%AFRIKAANSE OPSOMMING:…" @default.
• W2156815577 created "2016-06-24" @default.
• W2156815577 creator A5001072342 @default.
• W2156815577 creator A5081054179 @default.
• W2156815577 date "2015-04-01" @default.
• W2156815577 modified "2023-09-27" @default.
• W2156815577 title "In vitro and In vivo Characterization of Amyloliquecidin, a Novel Two- Component Lantibiotic Produced by Bacillus amyloliquefaciens" @default.
• W2156815577 cites W144957065 @default.
• W2156815577 cites W1547054036 @default.
• W2156815577 cites W1548366145 @default.
• W2156815577 cites W1601718706 @default.
• W2156815577 cites W1606628932 @default.
• W2156815577 cites W1607922172 @default.
• W2156815577 cites W1758794665 @default.
• W2156815577 cites W1798992768 @default.
• W2156815577 cites W1908065592 @default.
• W2156815577 cites W1939139620 @default.
• W2156815577 cites W1963494339 @default.
• W2156815577 cites W1964765214 @default.
• W2156815577 cites W1966664751 @default.
• W2156815577 cites W1968051013 @default.
• W2156815577 cites W1968125869 @default.
• W2156815577 cites W1970798262 @default.
• W2156815577 cites W1971075491 @default.
• W2156815577 cites W1972580525 @default.
• W2156815577 cites W1973641356 @default.
• W2156815577 cites W1974444010 @default.
• W2156815577 cites W1974541260 @default.
• W2156815577 cites W1974563120 @default.
• W2156815577 cites W1976014853 @default.
• W2156815577 cites W1977099733 @default.
• W2156815577 cites W1980595924 @default.
• W2156815577 cites W1981311183 @default.
• W2156815577 cites W1984683656 @default.
• W2156815577 cites W1986308133 @default.
• W2156815577 cites W1991474181 @default.
• W2156815577 cites W1996738507 @default.
• W2156815577 cites W1996785066 @default.
• W2156815577 cites W1997895087 @default.
• W2156815577 cites W2000821793 @default.
• W2156815577 cites W2004687702 @default.
• W2156815577 cites W2009097399 @default.
• W2156815577 cites W2011731141 @default.
• W2156815577 cites W2013424910 @default.
• W2156815577 cites W2013755857 @default.
• W2156815577 cites W2013756314 @default.
• W2156815577 cites W2013796387 @default.
• W2156815577 cites W2015869850 @default.
• W2156815577 cites W2016578240 @default.
• W2156815577 cites W2020559431 @default.
• W2156815577 cites W2020799333 @default.
• W2156815577 cites W2022004374 @default.
• W2156815577 cites W2022124341 @default.
• W2156815577 cites W2024128381 @default.
• W2156815577 cites W2031920409 @default.
• W2156815577 cites W2032136328 @default.
• W2156815577 cites W2034833171 @default.
• W2156815577 cites W2041958543 @default.
• W2156815577 cites W2042321138 @default.
• W2156815577 cites W2043051000 @default.
• W2156815577 cites W2043437490 @default.
• W2156815577 cites W2049730653 @default.
• W2156815577 cites W2049815243 @default.
• W2156815577 cites W2053819820 @default.
• W2156815577 cites W2054234131 @default.
• W2156815577 cites W2054967201 @default.
• W2156815577 cites W2055091410 @default.
• W2156815577 cites W2056174071 @default.
• W2156815577 cites W2059789891 @default.
• W2156815577 cites W2060051471 @default.
• W2156815577 cites W2061748348 @default.
• W2156815577 cites W2064618548 @default.
• W2156815577 cites W2065139121 @default.
• W2156815577 cites W2072966830 @default.
• W2156815577 cites W2073556554 @default.
• W2156815577 cites W2075892592 @default.
• W2156815577 cites W2079162636 @default.
• W2156815577 cites W2085805117 @default.
• W2156815577 cites W2085966015 @default.
• W2156815577 cites W2086057691 @default.
• W2156815577 cites W2087352312 @default.
• W2156815577 cites W2087993164 @default.
• W2156815577 cites W2088057731 @default.
• W2156815577 cites W2089535574 @default.
• W2156815577 cites W2098362401 @default.
• W2156815577 cites W2098515959 @default.
• W2156815577 cites W2099730013 @default.
• W2156815577 cites W2099839947 @default.
• W2156815577 cites W2100964796 @default.
• W2156815577 cites W2101169552 @default.
• W2156815577 cites W2104269235 @default.
• W2156815577 cites W2104675722 @default.
• W2156815577 cites W2109623513 @default.
• W2156815577 cites W2110693414 @default.
• W2156815577 cites W2111785687 @default.
• W2156815577 cites W2111899110 @default.
• W2156815577 cites W2115736733 @default.
• W2156815577 cites W2117720741 @default.
• W2156815577 cites W2118423669 @default.
• W2156815577 cites W2118991174 @default.

• next