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• W2156820997 abstract "ABSTRACT The genus Pseudomonas includes several species with an important biotechnological potential. Here we present a molecular approach for the analysis of the diversity of Pseudomonas spp. in aquatic samples based on the amplification of 16S rDNAs using taxon‐specific primers, followed by multienzymatic restrictions and subsequent screening of terminal restriction fragments using capillary electrophoresis. This method, tested on seawater, groundwater and aquatic sediments, allows the identification of Pseudomonas spp. in samples, in which culture‐based approaches failed in identifying their presence. The diversity of Pseudomonas spp. was very high in all samples investigated, with values higher than those reported in previous studies. The method was intercalibrated with Pseudomonas sp. isolates and allowed the distinction of different isolates. The possibility of identifying the taxonomic affiliation of the different isolates was tested with an “ in silico ” computer‐assisted analysis (TAP terminal restriction fragment length polymorphisms). PRACTICAL APPLICATIONS The method presented here allows the rapid and low‐cost identification of Pseudomonas spp., and an accurate determination of the diversity of this genus that can be carried out routinely in a large number of samples. This method has the potential to screen Pseudomonas libraries and can be applied in theframework of bioremediation and biomonitoring studies, thus potentially contributing to studies in the fields of environmental monitoring, microbial ecology and bioremediation." @default.
• W2156820997 created "2016-06-24" @default.
• W2156820997 creator A5029575829 @default.
• W2156820997 creator A5074863986 @default.
• W2156820997 date "2008-12-01" @default.
• W2156820997 modified "2023-10-16" @default.
• W2156820997 title "RAPID IDENTIFICATION OF<i>PSEUDOMONAS</i>SPP. FROM AQUATIC SAMPLES USING TERMINAL RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS" @default.
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• W2156820997 doi "https://doi.org/10.1111/j.1745-4581.2008.00141.x" @default.
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