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• W2156836664 abstract "Studies were undertaken to determine if changes in the amplitude of luteinizing hormone (LH) pulses that occur in response to changes in the frequency of gonadotropin-releasing hormone (GnRH) pulses are due to an alteration in the number of GnRH receptors. Ewes were ovariectomized (OVX) and the hypothalamus was disconnected from the pituitary (HPD). Ewes were then given pulses of GnRH at a frequency of 1/h or 1/3 h. Two control groups were included: OVX ewes not subjected to HPD, and HPD ewes that were not OVX. At the end of one week of treatment, blood samples were collected to determine the amplitude of LH pulses. The treated ewes were killed just before the next scheduled pulse of GnRH, and the content of LH and number of GnRH receptors were measured in each pituitary. The amplitude of LH pulses was highly correlated with the amount of LH in the pituitary gland (0 = 0.71, p < 0.01), and both LH content and pulse amplitude (mean + SEM) were higher in ewes receiving GnRH once per 3 h (189.7 ± 39.3 μg/pituitary, 10.3 ± 1.1 ng/ml, respectively) than in ewes receiving GnRH once per h (77.8 ± 11.4 μg/pituitary, 5.2 ± 1.3 ng/ml). The pituitary content of LH was highest in the OVX ewes (260.2 ± 57.4 μg/pituitary) and lowest in the nonpulsed HPD ewes (61.7 ± 51.2 μg/pituitary). The number of GnRH receptors was similar in all groups, and was not correlated with any other variable. From these data, we conclude that changes in LH pulse amplitude in response to changes in GnRH pulse frequency are more likely to be associated with the content of LH in the pituitary gland than with the number of GnRH receptors." @default.
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• W2156836664 date "1987-11-01" @default.
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• W2156836664 title "Pituitary Receptors for Gonadotropin-Releasing Hormone in Relation to Changes in Pituitary and Plasma Luteinizing Hormone in Ovariectomized-Hypothalamo Pituitary Disconnected Ewes. I. Effect of Changing Frequency of Gonadotropin-Releasing Hormone Pulses1" @default.
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• W2156836664 doi "https://doi.org/10.1095/biolreprod37.4.749" @default.
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