FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2156850933> ?p ?o ?g. }

• W2156850933 endingPage "1732" @default.
• W2156850933 startingPage "1721" @default.
• W2156850933 abstract "Human African trypanosomiasis is a neglected tropical disease caused by the protozoan parasite, Trypanosoma brucei. In the mammalian bloodstream, the trypanosome’s metabolism differs significantly from that of its host. For example, the parasite relies exclusively on glycolysis for energy source. Recently, computational and mathematical models of trypanosome metabolism have been generated to assist in understanding the parasite metabolism with the aim of facilitating drug development. Optimisation of these models requires quantitative information, including metabolite concentrations and/or metabolic fluxes that have been hitherto unavailable on a large scale. Here, we have implemented an LC–MS-based method that allows large scale quantification of metabolite levels by using U-13C-labelled E. coli extracts as internal standards. Known amounts of labelled E. coli extract were added into the parasite samples, as well as calibration standards, and used to obtain calibration curves enabling us to convert intensities into concentrations. This method allowed us to reliably quantify the changes of 43 intracellular metabolites and 32 extracellular metabolites in the medium over time. Based on the absolute quantification, we were able to compute consumption and production fluxes. These quantitative data can now be used to optimise computational models of parasite metabolism." @default.
• W2156850933 created "2016-06-24" @default.
• W2156850933 creator A5022220990 @default.
• W2156850933 creator A5064632708 @default.
• W2156850933 creator A5070145336 @default.
• W2156850933 creator A5071406353 @default.
• W2156850933 creator A5073175338 @default.
• W2156850933 date "2015-07-09" @default.
• W2156850933 modified "2023-09-27" @default.
• W2156850933 title "LC–MS-based absolute metabolite quantification: application to metabolic flux measurement in trypanosomes" @default.
• W2156850933 cites W1611854178 @default.
• W2156850933 cites W1679104356 @default.
• W2156850933 cites W1927064503 @default.
• W2156850933 cites W1966753532 @default.
• W2156850933 cites W1973543110 @default.
• W2156850933 cites W1976482941 @default.
• W2156850933 cites W1979015967 @default.
• W2156850933 cites W1979986507 @default.
• W2156850933 cites W1981831051 @default.
• W2156850933 cites W1991265914 @default.
• W2156850933 cites W2002729383 @default.
• W2156850933 cites W2004235182 @default.
• W2156850933 cites W2007281810 @default.
• W2156850933 cites W2007378834 @default.
• W2156850933 cites W2014311481 @default.
• W2156850933 cites W2018062352 @default.
• W2156850933 cites W2021123893 @default.
• W2156850933 cites W2022348939 @default.
• W2156850933 cites W2030396019 @default.
• W2156850933 cites W2030776070 @default.
• W2156850933 cites W2031013641 @default.
• W2156850933 cites W2032008503 @default.
• W2156850933 cites W2033109567 @default.
• W2156850933 cites W2036388292 @default.
• W2156850933 cites W2052075760 @default.
• W2156850933 cites W2055625001 @default.
• W2156850933 cites W2057685086 @default.
• W2156850933 cites W2057983756 @default.
• W2156850933 cites W2058685556 @default.
• W2156850933 cites W2060733138 @default.
• W2156850933 cites W2060974253 @default.
• W2156850933 cites W2063221437 @default.
• W2156850933 cites W2081123911 @default.
• W2156850933 cites W2081414531 @default.
• W2156850933 cites W2082706597 @default.
• W2156850933 cites W2087230327 @default.
• W2156850933 cites W2092504964 @default.
• W2156850933 cites W2092571535 @default.
• W2156850933 cites W2094820379 @default.
• W2156850933 cites W2104434818 @default.
• W2156850933 cites W2107332260 @default.
• W2156850933 cites W2112684164 @default.
• W2156850933 cites W2118252985 @default.
• W2156850933 cites W2119992992 @default.
• W2156850933 cites W2125899377 @default.
• W2156850933 cites W2130237363 @default.
• W2156850933 cites W2130323754 @default.
• W2156850933 cites W2136669997 @default.
• W2156850933 cites W2136978039 @default.
• W2156850933 cites W2140695271 @default.
• W2156850933 cites W2151877638 @default.
• W2156850933 cites W2155151971 @default.
• W2156850933 cites W2155447723 @default.
• W2156850933 cites W2157689062 @default.
• W2156850933 cites W2168360174 @default.
• W2156850933 cites W2168377403 @default.
• W2156850933 cites W2168590651 @default.
• W2156850933 cites W2170954647 @default.
• W2156850933 doi "https://doi.org/10.1007/s11306-015-0827-2" @default.
• W2156850933 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4605981" @default.
• W2156850933 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26491423" @default.
• W2156850933 hasPublicationYear "2015" @default.
• W2156850933 type Work @default.
• W2156850933 sameAs 2156850933 @default.
• W2156850933 citedByCount "33" @default.
• W2156850933 countsByYear W21568509332016 @default.
• W2156850933 countsByYear W21568509332017 @default.
• W2156850933 countsByYear W21568509332018 @default.
• W2156850933 countsByYear W21568509332019 @default.
• W2156850933 countsByYear W21568509332020 @default.
• W2156850933 countsByYear W21568509332021 @default.
• W2156850933 countsByYear W21568509332022 @default.
• W2156850933 countsByYear W21568509332023 @default.
• W2156850933 crossrefType "journal-article" @default.
• W2156850933 hasAuthorship W2156850933A5022220990 @default.
• W2156850933 hasAuthorship W2156850933A5064632708 @default.
• W2156850933 hasAuthorship W2156850933A5070145336 @default.
• W2156850933 hasAuthorship W2156850933A5071406353 @default.
• W2156850933 hasAuthorship W2156850933A5073175338 @default.
• W2156850933 hasBestOaLocation W21568509331 @default.
• W2156850933 hasConcept C104317684 @default.
• W2156850933 hasConcept C136764020 @default.
• W2156850933 hasConcept C159047783 @default.
• W2156850933 hasConcept C178790620 @default.
• W2156850933 hasConcept C185592680 @default.
• W2156850933 hasConcept C21565614 @default.
• W2156850933 hasConcept C2777477808 @default.
• W2156850933 hasConcept C2778143080 @default.

• next