FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2156863092> ?p ?o ?g. }

• W2156863092 endingPage "4834" @default.
• W2156863092 startingPage "4826" @default.
• W2156863092 abstract "LipL32 is the most abundant outer membrane protein from pathogenic Leptospira and has been shown to bind extracellular matrix (ECM) proteins as well as Ca(2+). Recent crystal structures have been obtained for the protein in the apo- and Ca(2+)-bound forms. In this work, we produced three LipL32 mutants (D163-168A, Q67A, and S247A) and evaluated their ability to interact with Ca(2+) and with ECM glycoproteins and human plasminogen. The D163-168A mutant modifies aspartate residues involved in Ca(2+) binding, whereas the other two modify residues in a cavity on the other side of the protein structure. Loss of calcium binding in the D163-D168A mutant was confirmed using intrinsic tryptophan fluorescence, circular dichroism, and thermal denaturation whereas the Q67A and S247A mutants presented the same Ca(2+) affinity as the wild-type protein. We then evaluated if Ca(2+) binding to LipL32 would be crucial for its interaction with collagen type IV and plasma proteins fibronectin and plasminogen. Surprisingly, the wild-type protein and all three mutants, including the D163-168A variant, bound to these ECM proteins with very similar affinities, both in the presence and absence of Ca(2+) ions. In conclusion, calcium binding to LipL32 may be important to stabilize the protein, but is not necessary to mediate interaction with host extracellular matrix proteins." @default.
• W2156863092 created "2016-06-24" @default.
• W2156863092 creator A5034695637 @default.
• W2156863092 creator A5066066519 @default.
• W2156863092 creator A5077606784 @default.
• W2156863092 creator A5079193460 @default.
• W2156863092 date "2012-02-01" @default.
• W2156863092 modified "2023-09-30" @default.
• W2156863092 title "Calcium Binding to Leptospira Outer Membrane Antigen LipL32 Is Not Necessary for Its Interaction with Plasma Fibronectin, Collagen Type IV, and Plasminogen" @default.
• W2156863092 cites W1489748911 @default.
• W2156863092 cites W1552033009 @default.
• W2156863092 cites W1941449561 @default.
• W2156863092 cites W1973027341 @default.
• W2156863092 cites W1982451112 @default.
• W2156863092 cites W1986055146 @default.
• W2156863092 cites W1986555537 @default.
• W2156863092 cites W1996732725 @default.
• W2156863092 cites W1997541124 @default.
• W2156863092 cites W2010800683 @default.
• W2156863092 cites W2021512395 @default.
• W2156863092 cites W2056073188 @default.
• W2156863092 cites W2058901986 @default.
• W2156863092 cites W2079830634 @default.
• W2156863092 cites W2080151150 @default.
• W2156863092 cites W2090163045 @default.
• W2156863092 cites W2096954815 @default.
• W2156863092 cites W2109186440 @default.
• W2156863092 cites W2114325890 @default.
• W2156863092 cites W2115743884 @default.
• W2156863092 cites W2117777941 @default.
• W2156863092 cites W2120497357 @default.
• W2156863092 cites W2122315722 @default.
• W2156863092 cites W2126003114 @default.
• W2156863092 cites W2128121050 @default.
• W2156863092 cites W2138250109 @default.
• W2156863092 cites W2148403797 @default.
• W2156863092 cites W2152141463 @default.
• W2156863092 cites W2152311433 @default.
• W2156863092 cites W2152936708 @default.
• W2156863092 cites W2156383963 @default.
• W2156863092 cites W2162469452 @default.
• W2156863092 cites W2162705236 @default.
• W2156863092 cites W2165754522 @default.
• W2156863092 cites W2166458547 @default.
• W2156863092 cites W2167417906 @default.
• W2156863092 doi "https://doi.org/10.1074/jbc.m111.277210" @default.
• W2156863092 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3281616" @default.
• W2156863092 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22147698" @default.
• W2156863092 hasPublicationYear "2012" @default.
• W2156863092 type Work @default.
• W2156863092 sameAs 2156863092 @default.
• W2156863092 citedByCount "16" @default.
• W2156863092 countsByYear W21568630922012 @default.
• W2156863092 countsByYear W21568630922013 @default.
• W2156863092 countsByYear W21568630922014 @default.
• W2156863092 countsByYear W21568630922015 @default.
• W2156863092 countsByYear W21568630922016 @default.
• W2156863092 countsByYear W21568630922020 @default.
• W2156863092 countsByYear W21568630922023 @default.
• W2156863092 crossrefType "journal-article" @default.
• W2156863092 hasAuthorship W2156863092A5034695637 @default.
• W2156863092 hasAuthorship W2156863092A5066066519 @default.
• W2156863092 hasAuthorship W2156863092A5077606784 @default.
• W2156863092 hasAuthorship W2156863092A5079193460 @default.
• W2156863092 hasBestOaLocation W21568630921 @default.
• W2156863092 hasConcept C104317684 @default.
• W2156863092 hasConcept C108625454 @default.
• W2156863092 hasConcept C122229402 @default.
• W2156863092 hasConcept C12554922 @default.
• W2156863092 hasConcept C133571119 @default.
• W2156863092 hasConcept C143065580 @default.
• W2156863092 hasConcept C178790620 @default.
• W2156863092 hasConcept C185592680 @default.
• W2156863092 hasConcept C189165786 @default.
• W2156863092 hasConcept C51639874 @default.
• W2156863092 hasConcept C519063684 @default.
• W2156863092 hasConcept C55493867 @default.
• W2156863092 hasConcept C86492073 @default.
• W2156863092 hasConcept C86803240 @default.
• W2156863092 hasConceptScore W2156863092C104317684 @default.
• W2156863092 hasConceptScore W2156863092C108625454 @default.
• W2156863092 hasConceptScore W2156863092C122229402 @default.
• W2156863092 hasConceptScore W2156863092C12554922 @default.
• W2156863092 hasConceptScore W2156863092C133571119 @default.
• W2156863092 hasConceptScore W2156863092C143065580 @default.
• W2156863092 hasConceptScore W2156863092C178790620 @default.
• W2156863092 hasConceptScore W2156863092C185592680 @default.
• W2156863092 hasConceptScore W2156863092C189165786 @default.
• W2156863092 hasConceptScore W2156863092C51639874 @default.
• W2156863092 hasConceptScore W2156863092C519063684 @default.
• W2156863092 hasConceptScore W2156863092C55493867 @default.
• W2156863092 hasConceptScore W2156863092C86492073 @default.
• W2156863092 hasConceptScore W2156863092C86803240 @default.
• W2156863092 hasIssue "7" @default.
• W2156863092 hasLocation W21568630921 @default.
• W2156863092 hasLocation W21568630922 @default.
• W2156863092 hasLocation W21568630923 @default.
• W2156863092 hasLocation W21568630924 @default.

• next