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• W2156866812 abstract "Mammalian cells possess two distinct types of DNA: the mitochondrial genome; and the nuclear chromosome. The former may be only 16.5 kbp in size. Although the mammalian mitochondrial genome is far smaller than the DNA cloned using Escherichia coli during the early 1990s, cloning of the first whole mammalian mitochondrial genome was not accomplished until 2005. The nuclear chromosome, which is too large to be cloned by any method, has been segmented into an Escherichia coli BAC vector, and is now available as a mouse BAC DNA library. For sophisticated genetic research, large, complicated and highly engineered DNA is required. However, current protocols rely too heavily on the E. coli molecular cloning system, and cannot meet all present research demands. In this chapter, an alternative novel cloning system is reviewed, termed Bacillus genome manipulation (BGM). The BGM system is not merely an auxiliary method but rather a crucial advance that satisfies nearly all requirements for a wide range of global applications. Attention will be focused on three BGM topics relevant to mouse genetics and genomics, with reference to recent studies. These topics include: cloning full-size mouse mitochondrial genomes; giant mouse chromosomal segments deliberately engineered for transgenic mice; and the long-term preservation of valuable DNA. The latter topic, though rarely discussed, is particularly important for controlling the costs of future uses of large DNA.Keywords:molecular cloning;metabolic engineering;gene assembly;BGM vector;OGAB method;long-term DNA reservoir" @default.
• W2156866812 created "2016-06-24" @default.
• W2156866812 creator A5061407924 @default.
• W2156866812 creator A5070148501 @default.
• W2156866812 date "2014-10-20" @default.
• W2156866812 modified "2023-09-24" @default.
• W2156866812 title "Production of Mitochondrial Genome and Chromosomal DNA Segments Highly Engineered for Use in Mouse Genetics by aBacillus subtilis-Based BGM Vector System" @default.
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• W2156866812 doi "https://doi.org/10.1002/3527600906.mcb.20120081" @default.
• W2156866812 hasPublicationYear "2014" @default.
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