FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2156876763> ?p ?o ?g. }

• W2156876763 endingPage "977" @default.
• W2156876763 startingPage "969" @default.
• W2156876763 abstract "Epithelial-mesenchymal interactions mediate aspects of normal lung growth and development and are important in the restoration of normal alveolar architecture after lung injury. To determine if fibroblasts are a source of soluble growth factors for alveolar type II cells, we investigated the effect of fibroblast-conditioned medium (CM) on alveolar type II cell DNA synthesis. Serum-free CM from confluent adult human lung fibroblasts was concentrated fivefold by lyophilization. Type II cells were isolated from adult rats by elastase dissociation and incubated with [3H]thymidine and varying dilutions of concentrated CM and serum from day 1 to 3 of culture. Stimulation of type II cell DNA synthesis by fibroblast-CM was maximal after 48 h of conditioning and required the presence of serum. The activity of the CM was eliminated by boiling and by treatment with trypsin, pepsin, or dithiothreitol and was additive with saturating concentrations of acidic fibroblast growth factor, epidermal growth factor, and insulin. The growth factor activity bound to heparin-Sepharose and was eluted with 0.6 and 1.0 M NaCl. Neutralizing antibody studies demonstrated that the primary mitogens isolated in the 0.6 and 1.0 M NaCl fractions were keratinocyte growth factor (KGF, fibroblast growth factor 7) and hepatocyte growth factor/scatter factor (HGF/SF), respectively. HGF/SF was demonstrated in the crude CM and KGF was detected in the 0.6 M NaCl eluent by immunoblotting. Northern blot analysis confirmed that the lung fibroblasts expressed both KGF and HGF/SF transcripts. Human recombinant KGF and HGF/SF induced a concentration- and serum-dependent increase in rat alveolar type II cell DNA synthesis. We conclude that adult human lung fibroblasts produce at least two soluble heparin-binding growth factors, KGF and HGF/SF, which promote DNA synthesis and proliferation of rat alveolar type II cells in primary culture. KGF and HGF/SF may be important stimuli for alveolar type II cell proliferation during lung growth and after lung injury." @default.
• W2156876763 created "2016-06-24" @default.
• W2156876763 creator A5009526225 @default.
• W2156876763 creator A5047197532 @default.
• W2156876763 creator A5053364502 @default.
• W2156876763 creator A5067641826 @default.
• W2156876763 creator A5083062288 @default.
• W2156876763 date "1993-08-01" @default.
• W2156876763 modified "2023-09-23" @default.
• W2156876763 title "Keratinocyte growth factor and hepatocyte growth factor/scatter factor are heparin-binding growth factors for alveolar type II cells in fibroblast-conditioned medium." @default.
• W2156876763 cites W1514636046 @default.
• W2156876763 cites W1519913430 @default.
• W2156876763 cites W1750535841 @default.
• W2156876763 cites W1754681212 @default.
• W2156876763 cites W1933888786 @default.
• W2156876763 cites W1964840302 @default.
• W2156876763 cites W1968927999 @default.
• W2156876763 cites W1971717847 @default.
• W2156876763 cites W1976562415 @default.
• W2156876763 cites W1980551449 @default.
• W2156876763 cites W1981268312 @default.
• W2156876763 cites W1982044793 @default.
• W2156876763 cites W1989109426 @default.
• W2156876763 cites W1989297142 @default.
• W2156876763 cites W1993335186 @default.
• W2156876763 cites W1994527446 @default.
• W2156876763 cites W1995299530 @default.
• W2156876763 cites W2004427074 @default.
• W2156876763 cites W2005487014 @default.
• W2156876763 cites W2005782838 @default.
• W2156876763 cites W2007904495 @default.
• W2156876763 cites W2010163851 @default.
• W2156876763 cites W2011321125 @default.
• W2156876763 cites W2013842796 @default.
• W2156876763 cites W2015811901 @default.
• W2156876763 cites W2018289835 @default.
• W2156876763 cites W2029587780 @default.
• W2156876763 cites W2039295539 @default.
• W2156876763 cites W2041705218 @default.
• W2156876763 cites W2042202567 @default.
• W2156876763 cites W2043624883 @default.
• W2156876763 cites W2049043712 @default.
• W2156876763 cites W2049891000 @default.
• W2156876763 cites W2051998135 @default.
• W2156876763 cites W2052644135 @default.
• W2156876763 cites W2054042846 @default.
• W2156876763 cites W2056971358 @default.
• W2156876763 cites W2060333964 @default.
• W2156876763 cites W2073292917 @default.
• W2156876763 cites W2074641624 @default.
• W2156876763 cites W2077750858 @default.
• W2156876763 cites W2077838938 @default.
• W2156876763 cites W2078753736 @default.
• W2156876763 cites W2085165496 @default.
• W2156876763 cites W2088775512 @default.
• W2156876763 cites W2099543104 @default.
• W2156876763 cites W2100837269 @default.
• W2156876763 cites W2101108802 @default.
• W2156876763 cites W2104856326 @default.
• W2156876763 cites W2113065332 @default.
• W2156876763 cites W2135006631 @default.
• W2156876763 cites W2147150467 @default.
• W2156876763 cites W2155493449 @default.
• W2156876763 cites W2165910165 @default.
• W2156876763 cites W2166859563 @default.
• W2156876763 cites W2307290500 @default.
• W2156876763 cites W2336697595 @default.
• W2156876763 cites W2340387843 @default.
• W2156876763 cites W2394621202 @default.
• W2156876763 cites W2402429692 @default.
• W2156876763 cites W2411135442 @default.
• W2156876763 cites W2413212242 @default.
• W2156876763 cites W2414741912 @default.
• W2156876763 cites W2415092273 @default.
• W2156876763 cites W2416038845 @default.
• W2156876763 cites W2416988240 @default.
• W2156876763 cites W2430336341 @default.
• W2156876763 cites W2473907594 @default.
• W2156876763 cites W49571376 @default.
• W2156876763 cites W83231304 @default.
• W2156876763 doi "https://doi.org/10.1172/jci116673" @default.
• W2156876763 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/294937" @default.
• W2156876763 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7688769" @default.
• W2156876763 hasPublicationYear "1993" @default.
• W2156876763 type Work @default.
• W2156876763 sameAs 2156876763 @default.
• W2156876763 citedByCount "295" @default.
• W2156876763 countsByYear W21568767632012 @default.
• W2156876763 countsByYear W21568767632013 @default.
• W2156876763 countsByYear W21568767632014 @default.
• W2156876763 countsByYear W21568767632015 @default.
• W2156876763 countsByYear W21568767632016 @default.
• W2156876763 countsByYear W21568767632017 @default.
• W2156876763 countsByYear W21568767632018 @default.
• W2156876763 countsByYear W21568767632019 @default.
• W2156876763 countsByYear W21568767632020 @default.
• W2156876763 countsByYear W21568767632021 @default.
• W2156876763 countsByYear W21568767632022 @default.

• next