FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2156896172> ?p ?o ?g. }

• W2156896172 endingPage "e103" @default.
• W2156896172 startingPage "e103" @default.
• W2156896172 abstract "The detection of double-stranded (ds) DNA by SYBR Green I (SG) is important in many molecular biology methods including gel electrophoresis, dsDNA quantification in solution and real-time PCR. Biophysical studies at defined dye/base pair ratios (dbprs) were used to determine the structure–property relationships that affect methods applying SG. These studies revealed the occurrence of intercalation, followed by surface binding at dbprs above ∼0.15. Only the latter led to a significant increase in fluorescence. Studies with poly(dA) · poly(dT) and poly(dG) · poly(dC) homopolymers showed sequence-specific binding of SG. Also, salts had a marked impact on SG fluorescence. We also noted binding of SG to single-stranded (ss) DNA, although SG/ssDNA fluorescence was at least ∼11-fold lower than with dsDNA. To perform these studies, we determined the structure of SG by mass spectrometry and NMR analysis to be [2-[ N -(3-dimethylaminopropyl)- N -propylamino]-4-[2,3-dihydro-3-methyl-(benzo-1,3-thiazol-2-yl)-methylidene]-1-phenyl-quinolinium]. For comparison, the structure of PicoGreen (PG) was also determined and is [2-[ N -bis-(3-dimethylaminopropyl)-amino]-4-[2,3-dihydro-3-methyl-(benzo-1,3-thiazol-2-yl)-methylidene]-1-phenyl-quinolinium] + . These structure–property relationships help in the design of methods that use SG, in particular dsDNA quantification in solution and real-time PCR." @default.
• W2156896172 created "2016-06-24" @default.
• W2156896172 creator A5002958420 @default.
• W2156896172 creator A5080194585 @default.
• W2156896172 creator A5083722969 @default.
• W2156896172 creator A5084516050 @default.
• W2156896172 date "2004-07-07" @default.
• W2156896172 modified "2023-10-16" @default.
• W2156896172 title "Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications" @default.
• W2156896172 cites W1569947224 @default.
• W2156896172 cites W1594653092 @default.
• W2156896172 cites W1642259399 @default.
• W2156896172 cites W1922793792 @default.
• W2156896172 cites W1965272410 @default.
• W2156896172 cites W1966853302 @default.
• W2156896172 cites W1970019291 @default.
• W2156896172 cites W1971989264 @default.
• W2156896172 cites W1974083655 @default.
• W2156896172 cites W1982211257 @default.
• W2156896172 cites W1987208051 @default.
• W2156896172 cites W1994000836 @default.
• W2156896172 cites W1995295345 @default.
• W2156896172 cites W1995933703 @default.
• W2156896172 cites W1996991816 @default.
• W2156896172 cites W1998699639 @default.
• W2156896172 cites W1999434307 @default.
• W2156896172 cites W2004006097 @default.
• W2156896172 cites W2014012173 @default.
• W2156896172 cites W2017757590 @default.
• W2156896172 cites W2017846996 @default.
• W2156896172 cites W2023862524 @default.
• W2156896172 cites W2028129119 @default.
• W2156896172 cites W2042435873 @default.
• W2156896172 cites W2043074618 @default.
• W2156896172 cites W2044161393 @default.
• W2156896172 cites W2053468384 @default.
• W2156896172 cites W2058446854 @default.
• W2156896172 cites W2058716517 @default.
• W2156896172 cites W2059424443 @default.
• W2156896172 cites W2061547309 @default.
• W2156896172 cites W2078716199 @default.
• W2156896172 cites W2081983728 @default.
• W2156896172 cites W2084608393 @default.
• W2156896172 cites W2097813604 @default.
• W2156896172 cites W2110317284 @default.
• W2156896172 cites W2116297079 @default.
• W2156896172 cites W2118615056 @default.
• W2156896172 cites W2146224715 @default.
• W2156896172 cites W2150705960 @default.
• W2156896172 cites W2155645094 @default.
• W2156896172 cites W2444620364 @default.
• W2156896172 cites W49147553 @default.
• W2156896172 doi "https://doi.org/10.1093/nar/gnh101" @default.
• W2156896172 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/484200" @default.
• W2156896172 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15249599" @default.
• W2156896172 hasPublicationYear "2004" @default.
• W2156896172 type Work @default.
• W2156896172 sameAs 2156896172 @default.
• W2156896172 citedByCount "676" @default.
• W2156896172 countsByYear W21568961722012 @default.
• W2156896172 countsByYear W21568961722013 @default.
• W2156896172 countsByYear W21568961722014 @default.
• W2156896172 countsByYear W21568961722015 @default.
• W2156896172 countsByYear W21568961722016 @default.
• W2156896172 countsByYear W21568961722017 @default.
• W2156896172 countsByYear W21568961722018 @default.
• W2156896172 countsByYear W21568961722019 @default.
• W2156896172 countsByYear W21568961722020 @default.
• W2156896172 countsByYear W21568961722021 @default.
• W2156896172 countsByYear W21568961722022 @default.
• W2156896172 countsByYear W21568961722023 @default.
• W2156896172 crossrefType "journal-article" @default.
• W2156896172 hasAuthorship W2156896172A5002958420 @default.
• W2156896172 hasAuthorship W2156896172A5080194585 @default.
• W2156896172 hasAuthorship W2156896172A5083722969 @default.
• W2156896172 hasAuthorship W2156896172A5084516050 @default.
• W2156896172 hasBestOaLocation W21568961721 @default.
• W2156896172 hasConcept C104317684 @default.
• W2156896172 hasConcept C107824862 @default.
• W2156896172 hasConcept C121332964 @default.
• W2156896172 hasConcept C137824038 @default.
• W2156896172 hasConcept C153911025 @default.
• W2156896172 hasConcept C178790620 @default.
• W2156896172 hasConcept C185581394 @default.
• W2156896172 hasConcept C185592680 @default.
• W2156896172 hasConcept C2780968160 @default.
• W2156896172 hasConcept C48023723 @default.
• W2156896172 hasConcept C552990157 @default.
• W2156896172 hasConcept C55493867 @default.
• W2156896172 hasConcept C62520636 @default.
• W2156896172 hasConcept C71240020 @default.
• W2156896172 hasConcept C86803240 @default.
• W2156896172 hasConcept C91881484 @default.
• W2156896172 hasConceptScore W2156896172C104317684 @default.
• W2156896172 hasConceptScore W2156896172C107824862 @default.
• W2156896172 hasConceptScore W2156896172C121332964 @default.
• W2156896172 hasConceptScore W2156896172C137824038 @default.
• W2156896172 hasConceptScore W2156896172C153911025 @default.

• next