FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2156898947> ?p ?o ?g. }

• W2156898947 endingPage "870" @default.
• W2156898947 startingPage "858" @default.
• W2156898947 abstract "The objective of this study was to define the physiologic needs of domestic cat embryos to facilitate development of a feline-specific culture medium. In a series of factorial experiments, in vivo-matured oocytes (n = 2040) from gonadotropin-treated domestic cats were inseminated in vitro to generate embryos (n = 1464) for culture. In the initial study, concentrations of NaCl (100.0 vs. 120.0 mM), KCl (4.0 vs. 8.0 mM), KH(2)PO(4) (0.25 vs. 1.0 mM), and the ratio of CaCl(2) to MgSO(4)-7H(2)O (1.0:2.0 mM vs. 2.0:1.0 mM) in the medium were evaluated during Days 1-6 (Day 0: oocyte recovery and in vitro fertilization [IVF]) of culture. Subsequent experiments assessed the effects of varying concentrations of carbohydrate (glucose, 1.5, 3.0, or 6.0 mM; l-lactate, 3.0, 6.0, or 12.0 mM; and pyruvate, 0.1 or 1.0 mM) and essential amino acids (EAAs; 0, 0.5, or 1.0x) in the medium during Days 1-3 and Days 3-6 of culture. Inclusion of vitamins (0 vs. 1.0x) and fetal calf serum (FCS; 0 vs. 5% [v/v]) in the medium also was evaluated during Days 3-6. Development and metabolism of IVF embryos on Day 3 or Day 6 were compared to age-matched in vivo embryos recovered from naturally mated queens. A feline-optimized culture medium (FOCM) was formulated based on these results (100.0 mM NaCl, 8.0 mM KCl, 1.0 mM KH(2)PO(4), 2.0 mM CaCl(2), 1.0 mM MgSO(4), 1.5 mM glucose, 6.0 mM L-lactate, 0.1 mM pyruvate, and 0x EAAs with 25.0 mM NaHCO(3), 1.0 mM alanyl-glutamine, 0.1 mM taurine, and 1.0x nonessential amino acids) with 0.4% (w/v) BSA from Days 0-3 and 5% FCS from Days 3-6. Using this medium, ~70% of cleaved embryos developed into blastocysts with profiles of carbohydrate metabolism similar to in vivo embryos. Our results suggest that feline embryos have stage-specific responses to carbohydrates and are sensitive to EAAs but are still reliant on one or more unidentified components of FCS for optimal blastocyst development." @default.
• W2156898947 created "2016-06-24" @default.
• W2156898947 creator A5003287588 @default.
• W2156898947 creator A5008649964 @default.
• W2156898947 creator A5048905643 @default.
• W2156898947 creator A5049127230 @default.
• W2156898947 creator A5053733240 @default.
• W2156898947 creator A5080469978 @default.
• W2156898947 creator A5088591275 @default.
• W2156898947 date "2007-05-01" @default.
• W2156898947 modified "2023-09-26" @default.
• W2156898947 title "Toward a Feline-Optimized Culture Medium: Impact of Ions, Carbohydrates, Essential Amino Acids, Vitamins, and Serum on Development and Metabolism of In Vitro Fertilization-Derived Feline Embryos Relative to Embryos Grown In Vivo1" @default.
• W2156898947 cites W1511989474 @default.
• W2156898947 cites W1513615956 @default.
• W2156898947 cites W1522030921 @default.
• W2156898947 cites W1546549208 @default.
• W2156898947 cites W1547572148 @default.
• W2156898947 cites W1551173276 @default.
• W2156898947 cites W1882711636 @default.
• W2156898947 cites W1980055551 @default.
• W2156898947 cites W1982606879 @default.
• W2156898947 cites W1984545842 @default.
• W2156898947 cites W1989727348 @default.
• W2156898947 cites W1991965826 @default.
• W2156898947 cites W1992362754 @default.
• W2156898947 cites W2001787785 @default.
• W2156898947 cites W2004248860 @default.
• W2156898947 cites W2012268463 @default.
• W2156898947 cites W2015976889 @default.
• W2156898947 cites W2016106800 @default.
• W2156898947 cites W2016738037 @default.
• W2156898947 cites W2018302600 @default.
• W2156898947 cites W2020944325 @default.
• W2156898947 cites W2022205071 @default.
• W2156898947 cites W2023959771 @default.
• W2156898947 cites W2024369133 @default.
• W2156898947 cites W2024691487 @default.
• W2156898947 cites W2024764076 @default.
• W2156898947 cites W2035822356 @default.
• W2156898947 cites W2041371683 @default.
• W2156898947 cites W2041884084 @default.
• W2156898947 cites W2042689896 @default.
• W2156898947 cites W2044587101 @default.
• W2156898947 cites W2049846676 @default.
• W2156898947 cites W2050735007 @default.
• W2156898947 cites W2051659348 @default.
• W2156898947 cites W2055096443 @default.
• W2156898947 cites W2057655236 @default.
• W2156898947 cites W2060235056 @default.
• W2156898947 cites W2062048112 @default.
• W2156898947 cites W2062920544 @default.
• W2156898947 cites W2063404951 @default.
• W2156898947 cites W2064526680 @default.
• W2156898947 cites W2065062325 @default.
• W2156898947 cites W2065154417 @default.
• W2156898947 cites W2066707862 @default.
• W2156898947 cites W2071264878 @default.
• W2156898947 cites W2076949184 @default.
• W2156898947 cites W2081512326 @default.
• W2156898947 cites W2082445072 @default.
• W2156898947 cites W2090063008 @default.
• W2156898947 cites W2091477180 @default.
• W2156898947 cites W2093066950 @default.
• W2156898947 cites W2093484517 @default.
• W2156898947 cites W2099912955 @default.
• W2156898947 cites W2104040825 @default.
• W2156898947 cites W2106610882 @default.
• W2156898947 cites W2107645087 @default.
• W2156898947 cites W2108522941 @default.
• W2156898947 cites W2109602352 @default.
• W2156898947 cites W2117247384 @default.
• W2156898947 cites W2121011454 @default.
• W2156898947 cites W2121230264 @default.
• W2156898947 cites W2121930396 @default.
• W2156898947 cites W2122529144 @default.
• W2156898947 cites W2129844850 @default.
• W2156898947 cites W2133073488 @default.
• W2156898947 cites W2135566161 @default.
• W2156898947 cites W2137906199 @default.
• W2156898947 cites W2139789898 @default.
• W2156898947 cites W2146047318 @default.
• W2156898947 cites W2146883159 @default.
• W2156898947 cites W2147536097 @default.
• W2156898947 cites W2147647873 @default.
• W2156898947 cites W2150784261 @default.
• W2156898947 cites W2155436742 @default.
• W2156898947 cites W2160394791 @default.
• W2156898947 cites W2162480880 @default.
• W2156898947 cites W2163356262 @default.
• W2156898947 cites W2166885691 @default.
• W2156898947 cites W2169649306 @default.
• W2156898947 cites W2169693407 @default.
• W2156898947 cites W2169770145 @default.
• W2156898947 cites W2182830251 @default.
• W2156898947 cites W2277225380 @default.
• W2156898947 cites W2312753901 @default.
• W2156898947 cites W2404013828 @default.
• W2156898947 cites W254700600 @default.

• next