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• W2157140330 abstract "ABSTRACT Ralstonia eutropha JMP134(pJP4) degrades 3-chlorobenzoate (3-CB) by using two not completely isofunctional, pJP4-encoded chlorocatechol degradation gene clusters, tfdC I D I E I F I and tfdD II C II E II F II . Introduction of several copies of each gene cluster into R. eutropha JMP222, which lacks pJP4 and thus accumulates chlorocatechols from 3-CB, allows the derivatives to grow in this substrate. However, JMP222 derivatives containing one chromosomal copy of each cluster did not grow in 3-CB. The failure to grow in 3-CB was the result of accumulation of chlorocatechols due to the limiting activity of chlorocatechol 1,2-dioxygenase (TfdC), the first enzyme in the chlorocatechol degradation pathway. Micromolar concentrations of 3- and 4-chlorocatechol inhibited the growth of strains JMP134 and JMP222 in benzoate, and cells of strain JMP222 exposed to 3 mM 3-CB exhibited a 2-order-of-magnitude decrease in viability. This toxicity effect was not observed with strain JMP222 harboring multiple copies of the tfdC I gene, and the derivative of strain JMP222 containing tfdC I D I E I F I plus multiple copies of the tfdC I gene could efficiently grow in 3-CB. In addition, tfdC I and tfdC II gene mutants of strain JMP134 exhibited no growth and impaired growth in 3-CB, respectively. The introduction into strain JMP134 of the xylS - xylXYZL genes, encoding a broad-substrate-range benzoate 1,2-dioxygenase system and thus increasing the transformation of 3-CB into chlorocatechols, resulted in derivatives that exhibited a sharp decrease in the ability to grow in 3-CB. These observations indicate that the dosage of chlorocatechol-transforming genes is critical for growth in 3-CB. This effect depends on a delicate balance between chlorocatechol-producing and chlorocatechol-consuming reactions." @default.
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• W2157140330 date "2003-03-01" @default.
• W2157140330 modified "2023-09-25" @default.
• W2157140330 title "Efficient Turnover of Chlorocatechols Is Essential for Growth of <i>Ralstonia eutropha</i> JMP134(pJP4) in 3-Chlorobenzoic Acid" @default.
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• W2157140330 doi "https://doi.org/10.1128/jb.185.5.1534-1542.2003" @default.
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