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- W2157172292 abstract "Objective: To determine the presence of the platelet-activating factor (PAF) receptor on human spermatozoa.Design: Prospective analysis.Setting: University-based reproductive biology laboratory.Patient(s): Spermatozoa obtained from men undergoing routine semen analysis.Intervention(s): Spermatozoa (n = 10) were exposed to PAF, sheep anti-PAF antibody, and fluorescein isothiocyanate–conjugated rabbit antisheep antibody, and then evaluated by fluorescent microscopy.Main Outcome Measure(s): Assessment of fluorescent intensity at four locations on the spermatozoa (tail, midpiece, proximal head, and acrosome region).Result(s): Immunofluorescence demonstrated the presence of the PAF receptor on human spermatozoa. The PAF receptor was most prevalent at two sites on the spermatozoa: the midpiece and the proximal head.Conclusion(s): The PAF receptor is present on human spermatozoa. Platelet-activating factor may affect the motility of spermatozoa through a receptor-mediated mechanism at the midpiece and/or proximal head." @default.
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- W2157172292 date "1999-05-01" @default.
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- W2157172292 title "Immunofluorescent evidence of the platelet-activating factor receptor on human spermatozoa" @default.
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- W2157172292 doi "https://doi.org/10.1016/s0015-0282(99)00096-5" @default.
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