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• W2157238139 abstract "This work analysed factors that influence the induction of viable but nonculturable (VBNC) state in the common enteric pathogen, Vibrio parahaemolyticus. The susceptibility of the VBNC cells to environmental stresses was investigated.Bacterium was cultured in tryptic soy broth-3% NaCl medium, shifted to a nutrient-free Morita mineral salt-0.5% NaCl medium (pH 7.8) and further incubated at 4 degrees C in a static state to induce the VBNC state in 28-35 days. The culturability and viability of the cells were monitored by the plate count method and the Bac Light viable count method, respectively. Cells grown at the optimum growth temperature and in the exponential phase better induced the VBNC state than those grown at low temperature and in the stationary phase. Low salinity of the medium crucially and markedly shortened the induction period. The VBNC cells were highly resistant to thermal (42, 47 degrees C), low salinity (0% NaCl), or acid (pH 4.0) inactivation.Optimal conditions for inducing VBNC V. parahaemolyticus were reported. The increase in resistance of VBNC V. parahaemolyticus to thermal, low salinity and acidic inactivation verified that this state is entered as part of a survival strategy in an adverse environment.The methods for inducing VBNC V. parahaemolyticus in a markedly short time will facilitate further physiological and pathological study. The enhanced stress resistance of the VBNC cells should attract attention to the increased risk presented by this pathogen in food." @default.
• W2157238139 created "2016-06-24" @default.
• W2157238139 creator A5034665433 @default.
• W2157238139 creator A5085183868 @default.
• W2157238139 date "2004-02-01" @default.
• W2157238139 modified "2023-10-13" @default.
• W2157238139 title "Induction of viable but nonculturable state in Vibrio parahaemolyticus and its susceptibility to environmental stresses" @default.
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• W2157238139 doi "https://doi.org/10.1046/j.1365-2672.2004.02166.x" @default.
• W2157238139 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14723697" @default.
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