Matches in SemOpenAlex for { <https://semopenalex.org/work/W2157304833> ?p ?o ?g. }
- W2157304833 endingPage "1003" @default.
- W2157304833 startingPage "991" @default.
- W2157304833 abstract "Several medicinal plants that stimulate glucose uptake in skeletal muscle cells were identified from among species used by the Cree of Eeyou Istchee of northern Quebec to treat symptoms of diabetes. This study aimed to elucidate the mechanism of action of one of these products, the berries of Vaccinium vitis idaea, as well as to isolate and identify its active constituents using a classical bioassay-guided fractionation approach. Western immunoblot analysis in C2C12 muscle cells revealed that the ethanol extract of the berries stimulated the insulin-independent AMP-activated protein kinase (AMPK) pathway. The extract mildly inhibited ADP-stimulated oxygen consumption in isolated mitochondria, an effect consistent with metabolic stress and the ensuing stimulation of AMPK. This mechanism is highly analogous to that of Metformin. Fractionation guided by glucose uptake activity resulted in the isolation of ten compounds. The two most active, quercetin-3-O-glycosides, enhanced glucose uptake by 38-59% (50 muM; 18 h treatment) in the absence of insulin. Quercetin aglycone, a minor constituent, stimulated uptake by 37%. The quercetin glycosides and the aglycone stimulated the AMPK pathway at concentrations of 25-100 muM, but only the aglycone inhibited ATP synthase in isolated mitochondria (by 34 and 79% at 25 and 100 muM, respectively). This discrepancy suggests that the activity of the glycosides may require hydrolysis to the aglycone form. These findings indicate that quercetin and quercetin 3-O-glycosides are responsible for the antidiabetic activity of V. vitis crude berry extract mediated by AMPK. These common plant products may thus have potential applications for the prevention and treatment of insulin resistance and other metabolic diseases." @default.
- W2157304833 created "2016-06-24" @default.
- W2157304833 creator A5010990822 @default.
- W2157304833 creator A5014650672 @default.
- W2157304833 creator A5022082218 @default.
- W2157304833 creator A5023813377 @default.
- W2157304833 creator A5028321187 @default.
- W2157304833 creator A5032906731 @default.
- W2157304833 creator A5054614804 @default.
- W2157304833 creator A5054999608 @default.
- W2157304833 creator A5066046474 @default.
- W2157304833 creator A5089467311 @default.
- W2157304833 date "2010-01-19" @default.
- W2157304833 modified "2023-10-17" @default.
- W2157304833 title "Stimulation of AMP-activated protein kinase and enhancement of basal glucose uptake in muscle cells by quercetin and quercetin glycosides, active principles of the antidiabetic medicinal plant Vaccinium vitis-idaea" @default.
- W2157304833 cites W1538613429 @default.
- W2157304833 cites W1810681069 @default.
- W2157304833 cites W1838677832 @default.
- W2157304833 cites W1880875467 @default.
- W2157304833 cites W1965720652 @default.
- W2157304833 cites W1969261151 @default.
- W2157304833 cites W1969826464 @default.
- W2157304833 cites W1973057304 @default.
- W2157304833 cites W1975955075 @default.
- W2157304833 cites W1997330696 @default.
- W2157304833 cites W2003892126 @default.
- W2157304833 cites W2007415406 @default.
- W2157304833 cites W2012390470 @default.
- W2157304833 cites W2016466318 @default.
- W2157304833 cites W2018095250 @default.
- W2157304833 cites W2026248990 @default.
- W2157304833 cites W2028159480 @default.
- W2157304833 cites W2028843992 @default.
- W2157304833 cites W2037998901 @default.
- W2157304833 cites W2042065066 @default.
- W2157304833 cites W2057832887 @default.
- W2157304833 cites W2058073766 @default.
- W2157304833 cites W2059263158 @default.
- W2157304833 cites W2064781179 @default.
- W2157304833 cites W2075763304 @default.
- W2157304833 cites W2103450921 @default.
- W2157304833 cites W2106324494 @default.
- W2157304833 cites W2107476304 @default.
- W2157304833 cites W2111667034 @default.
- W2157304833 cites W2115245505 @default.
- W2157304833 cites W2117680364 @default.
- W2157304833 cites W2119950193 @default.
- W2157304833 cites W2125306830 @default.
- W2157304833 cites W2128627308 @default.
- W2157304833 cites W2138226825 @default.
- W2157304833 cites W2141273174 @default.
- W2157304833 cites W2153464870 @default.
- W2157304833 cites W2158905364 @default.
- W2157304833 cites W2164933864 @default.
- W2157304833 cites W2490817116 @default.
- W2157304833 cites W2591894476 @default.
- W2157304833 cites W4241876655 @default.
- W2157304833 cites W70400069 @default.
- W2157304833 doi "https://doi.org/10.1002/mnfr.200900218" @default.
- W2157304833 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20087853" @default.
- W2157304833 hasPublicationYear "2010" @default.
- W2157304833 type Work @default.
- W2157304833 sameAs 2157304833 @default.
- W2157304833 citedByCount "167" @default.
- W2157304833 countsByYear W21573048332012 @default.
- W2157304833 countsByYear W21573048332013 @default.
- W2157304833 countsByYear W21573048332014 @default.
- W2157304833 countsByYear W21573048332015 @default.
- W2157304833 countsByYear W21573048332016 @default.
- W2157304833 countsByYear W21573048332017 @default.
- W2157304833 countsByYear W21573048332018 @default.
- W2157304833 countsByYear W21573048332019 @default.
- W2157304833 countsByYear W21573048332020 @default.
- W2157304833 countsByYear W21573048332021 @default.
- W2157304833 countsByYear W21573048332022 @default.
- W2157304833 countsByYear W21573048332023 @default.
- W2157304833 crossrefType "journal-article" @default.
- W2157304833 hasAuthorship W2157304833A5010990822 @default.
- W2157304833 hasAuthorship W2157304833A5014650672 @default.
- W2157304833 hasAuthorship W2157304833A5022082218 @default.
- W2157304833 hasAuthorship W2157304833A5023813377 @default.
- W2157304833 hasAuthorship W2157304833A5028321187 @default.
- W2157304833 hasAuthorship W2157304833A5032906731 @default.
- W2157304833 hasAuthorship W2157304833A5054614804 @default.
- W2157304833 hasAuthorship W2157304833A5054999608 @default.
- W2157304833 hasAuthorship W2157304833A5066046474 @default.
- W2157304833 hasAuthorship W2157304833A5089467311 @default.
- W2157304833 hasConcept C134018914 @default.
- W2157304833 hasConcept C184235292 @default.
- W2157304833 hasConcept C185592680 @default.
- W2157304833 hasConcept C194671627 @default.
- W2157304833 hasConcept C2777866211 @default.
- W2157304833 hasConcept C2778004101 @default.
- W2157304833 hasConcept C2779306644 @default.
- W2157304833 hasConcept C2779396346 @default.
- W2157304833 hasConcept C2780124434 @default.
- W2157304833 hasConcept C2781263971 @default.
- W2157304833 hasConcept C55493867 @default.