FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2157315543> ?p ?o ?g. }

• W2157315543 endingPage "108" @default.
• W2157315543 startingPage "100" @default.
• W2157315543 abstract "Neutral drift occurring over millions or billions of years results in substantial sequence divergence among enzymes that catalyze the same reaction. Although natural selection maintains the primary activity of orthologous enzymes, there is, by definition, no selective pressure to maintain physiologically irrelevant promiscuous activities. Thus, the levels and the evolvabilities of promiscuous activities may vary among orthologous enzymes. Consistent with this expectation, we have found that the levels of a promiscuous activity in nine gamma-glutamyl phosphate reductase (ProA) orthologs vary by about 50-fold. Remarkably, a single amino acid change from Glu to Ala near the active site appeared to be critical for improvement of the promiscuous activity in every ortholog. The effects of this change varied dramatically. The improvement in the promiscuous activity varied from 50- to 770-fold, and, importantly, was not correlated with the initial level of the promiscuous activity. The decrease in the original activity varied from 190- to 2,100-fold. These results suggest that evolution of a novel enzyme may be possible in some microbes, but not in others. Further, these results underscore the importance of using multiple orthologs as starting points for directed evolution of novel enzyme activities." @default.
• W2157315543 created "2016-06-24" @default.
• W2157315543 creator A5001717910 @default.
• W2157315543 creator A5014865423 @default.
• W2157315543 creator A5044612854 @default.
• W2157315543 creator A5051987215 @default.
• W2157315543 date "2014-09-21" @default.
• W2157315543 modified "2023-09-26" @default.
• W2157315543 title "Differential Effects of a Mutation on the Normal and Promiscuous Activities of Orthologs: Implications for Natural and Directed Evolution" @default.
• W2157315543 cites W1610108086 @default.
• W2157315543 cites W1961837067 @default.
• W2157315543 cites W1968796137 @default.
• W2157315543 cites W1972722145 @default.
• W2157315543 cites W1980363424 @default.
• W2157315543 cites W2003890287 @default.
• W2157315543 cites W2011109781 @default.
• W2157315543 cites W2016666153 @default.
• W2157315543 cites W2020593965 @default.
• W2157315543 cites W2024722087 @default.
• W2157315543 cites W2085277871 @default.
• W2157315543 cites W2087980904 @default.
• W2157315543 cites W2091805387 @default.
• W2157315543 cites W2109441358 @default.
• W2157315543 cites W2113573267 @default.
• W2157315543 cites W2115645840 @default.
• W2157315543 cites W2123769421 @default.
• W2157315543 cites W2142962510 @default.
• W2157315543 cites W2144499617 @default.
• W2157315543 cites W2146473657 @default.
• W2157315543 cites W2152603063 @default.
• W2157315543 cites W2317385382 @default.
• W2157315543 doi "https://doi.org/10.1093/molbev/msu271" @default.
• W2157315543 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4271523" @default.
• W2157315543 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25246702" @default.
• W2157315543 hasPublicationYear "2014" @default.
• W2157315543 type Work @default.
• W2157315543 sameAs 2157315543 @default.
• W2157315543 citedByCount "60" @default.
• W2157315543 countsByYear W21573155432015 @default.
• W2157315543 countsByYear W21573155432016 @default.
• W2157315543 countsByYear W21573155432017 @default.
• W2157315543 countsByYear W21573155432018 @default.
• W2157315543 countsByYear W21573155432019 @default.
• W2157315543 countsByYear W21573155432020 @default.
• W2157315543 countsByYear W21573155432021 @default.
• W2157315543 countsByYear W21573155432022 @default.
• W2157315543 countsByYear W21573155432023 @default.
• W2157315543 crossrefType "journal-article" @default.
• W2157315543 hasAuthorship W2157315543A5001717910 @default.
• W2157315543 hasAuthorship W2157315543A5014865423 @default.
• W2157315543 hasAuthorship W2157315543A5044612854 @default.
• W2157315543 hasAuthorship W2157315543A5051987215 @default.
• W2157315543 hasBestOaLocation W21573155431 @default.
• W2157315543 hasConcept C104317684 @default.
• W2157315543 hasConcept C143065580 @default.
• W2157315543 hasConcept C154945302 @default.
• W2157315543 hasConcept C166393388 @default.
• W2157315543 hasConcept C181199279 @default.
• W2157315543 hasConcept C2777949028 @default.
• W2157315543 hasConcept C2908542902 @default.
• W2157315543 hasConcept C2988562018 @default.
• W2157315543 hasConcept C41008148 @default.
• W2157315543 hasConcept C501734568 @default.
• W2157315543 hasConcept C54355233 @default.
• W2157315543 hasConcept C55493867 @default.
• W2157315543 hasConcept C75268714 @default.
• W2157315543 hasConcept C78458016 @default.
• W2157315543 hasConcept C81917197 @default.
• W2157315543 hasConcept C86803240 @default.
• W2157315543 hasConcept C90132467 @default.
• W2157315543 hasConcept C9418097 @default.
• W2157315543 hasConceptScore W2157315543C104317684 @default.
• W2157315543 hasConceptScore W2157315543C143065580 @default.
• W2157315543 hasConceptScore W2157315543C154945302 @default.
• W2157315543 hasConceptScore W2157315543C166393388 @default.
• W2157315543 hasConceptScore W2157315543C181199279 @default.
• W2157315543 hasConceptScore W2157315543C2777949028 @default.
• W2157315543 hasConceptScore W2157315543C2908542902 @default.
• W2157315543 hasConceptScore W2157315543C2988562018 @default.
• W2157315543 hasConceptScore W2157315543C41008148 @default.
• W2157315543 hasConceptScore W2157315543C501734568 @default.
• W2157315543 hasConceptScore W2157315543C54355233 @default.
• W2157315543 hasConceptScore W2157315543C55493867 @default.
• W2157315543 hasConceptScore W2157315543C75268714 @default.
• W2157315543 hasConceptScore W2157315543C78458016 @default.
• W2157315543 hasConceptScore W2157315543C81917197 @default.
• W2157315543 hasConceptScore W2157315543C86803240 @default.
• W2157315543 hasConceptScore W2157315543C90132467 @default.
• W2157315543 hasConceptScore W2157315543C9418097 @default.
• W2157315543 hasIssue "1" @default.
• W2157315543 hasLocation W21573155431 @default.
• W2157315543 hasLocation W21573155432 @default.
• W2157315543 hasLocation W21573155433 @default.
• W2157315543 hasLocation W21573155434 @default.
• W2157315543 hasOpenAccess W2157315543 @default.
• W2157315543 hasPrimaryLocation W21573155431 @default.
• W2157315543 hasRelatedWork W2157175495 @default.
• W2157315543 hasRelatedWork W2157315543 @default.

• next