FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2157328736> ?p ?o ?g. }

• W2157328736 abstract "To understand the expression of genes involved in different complement pathways in the retina and retinal pigment epithelium (RPE)/choroid under physiologic conditions and how their expression is regulated by inflammatory cytokines.The expression of complement components of the classical pathway (CP), mannose-binding lectin (MBL) pathway, alternative pathway (AP), and terminal pathway in the retina and RPE/choroid was determined by conventional reverse transcription polymerase chain reaction (RT-PCR). The effect of inflammatory cytokines, tumor necrosis factor-alpha (TNF-α, 20 ng/ml), interleukin (IL)-6 (10 ng/ml), interferon-gamma (IFN-γ, 100 ng/ml) or lipopolysaccharides (LPS, 1 μg/ml) on the expression of these complement component genes was tested in vitro in primary cultured RPE cells and a microglial cell line (BV2 cells) and quantified by real-time RT-PCR.In the CP, complements C1qb, C1r, C1s, C2, and C4 were constitutively expressed by retina and RPE/choroid. Complement factor H and factor B of the AP as well as C3 were also detected in the retinal and RPE/choroidal tissues. In the MBL pathway, low levels of mannose-binding lectin (MBL)-associated serine protease (MASP)-1 in the retina and RPE/choroid and MASP2L in the retina were detected. Other components, including mannose-binding lectin 1 (MBL1), mannose-binding lectin 2 (MBL2), complement factor I (CFI), complement component 5 (C5) and complement factor H-related protein 1 (CFHR1), were not detected in either the retina or the RPE/choroid. The expression of CP- and AP-complement component genes in RPE and microglial cells was upregulated by interferon (IFN)-γ treatment. Treatment with TNF-α selectively upregulated the expression of C1s and C3 genes but downregulated complement factor H gene expression in RPE and microglial cells. The expression of genes involved in the MBL pathway was not affected by the inflammatory cytokines tested in this study.Retina and RPE/choroid express a variety of complement components that are involved mainly in the CP and AP. RPE and microglial cells are the main sources of retinal complement gene expression. Retinal complement gene expression is regulated by inflammatory cytokines, such as IFN-γ and TNF-α." @default.
• W2157328736 created "2016-06-24" @default.
• W2157328736 creator A5022116941 @default.
• W2157328736 creator A5027358797 @default.
• W2157328736 creator A5046784348 @default.
• W2157328736 date "2011-01-01" @default.
• W2157328736 modified "2023-09-26" @default.
• W2157328736 title "Complement gene expression and regulation in mouse retina and retinal pigment epithelium/choroid." @default.
• W2157328736 cites W1504400614 @default.
• W2157328736 cites W1521642954 @default.
• W2157328736 cites W1570453996 @default.
• W2157328736 cites W1593976080 @default.
• W2157328736 cites W192994596 @default.
• W2157328736 cites W1950453196 @default.
• W2157328736 cites W1964245315 @default.
• W2157328736 cites W1966896477 @default.
• W2157328736 cites W1976590223 @default.
• W2157328736 cites W1984186519 @default.
• W2157328736 cites W1984194241 @default.
• W2157328736 cites W1984362205 @default.
• W2157328736 cites W1989747234 @default.
• W2157328736 cites W1990237608 @default.
• W2157328736 cites W1993057071 @default.
• W2157328736 cites W1996470116 @default.
• W2157328736 cites W1998219965 @default.
• W2157328736 cites W2001121985 @default.
• W2157328736 cites W2008569472 @default.
• W2157328736 cites W2011361113 @default.
• W2157328736 cites W2019432894 @default.
• W2157328736 cites W2022799658 @default.
• W2157328736 cites W2028729697 @default.
• W2157328736 cites W2034060753 @default.
• W2157328736 cites W2036984054 @default.
• W2157328736 cites W2044753638 @default.
• W2157328736 cites W2044878909 @default.
• W2157328736 cites W2050568671 @default.
• W2157328736 cites W2051915414 @default.
• W2157328736 cites W2052647020 @default.
• W2157328736 cites W2056617843 @default.
• W2157328736 cites W2056951760 @default.
• W2157328736 cites W2059000778 @default.
• W2157328736 cites W2066108450 @default.
• W2157328736 cites W2078209460 @default.
• W2157328736 cites W2078901795 @default.
• W2157328736 cites W2089217040 @default.
• W2157328736 cites W2089275254 @default.
• W2157328736 cites W2096107242 @default.
• W2157328736 cites W2097802875 @default.
• W2157328736 cites W2100219076 @default.
• W2157328736 cites W2107521022 @default.
• W2157328736 cites W2110068074 @default.
• W2157328736 cites W2117324044 @default.
• W2157328736 cites W2139332576 @default.
• W2157328736 cites W2153596758 @default.
• W2157328736 cites W2162838839 @default.
• W2157328736 cites W2994150204 @default.
• W2157328736 cites W77072218 @default.
• W2157328736 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3123163" @default.
• W2157328736 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21738388" @default.
• W2157328736 hasPublicationYear "2011" @default.
• W2157328736 type Work @default.
• W2157328736 sameAs 2157328736 @default.
• W2157328736 citedByCount "41" @default.
• W2157328736 countsByYear W21573287362012 @default.
• W2157328736 countsByYear W21573287362014 @default.
• W2157328736 countsByYear W21573287362015 @default.
• W2157328736 countsByYear W21573287362016 @default.
• W2157328736 countsByYear W21573287362017 @default.
• W2157328736 countsByYear W21573287362018 @default.
• W2157328736 countsByYear W21573287362019 @default.
• W2157328736 countsByYear W21573287362021 @default.
• W2157328736 countsByYear W21573287362022 @default.
• W2157328736 countsByYear W21573287362023 @default.
• W2157328736 crossrefType "journal-article" @default.
• W2157328736 hasAuthorship W2157328736A5022116941 @default.
• W2157328736 hasAuthorship W2157328736A5027358797 @default.
• W2157328736 hasAuthorship W2157328736A5046784348 @default.
• W2157328736 hasConcept C111684460 @default.
• W2157328736 hasConcept C136015124 @default.
• W2157328736 hasConcept C138873322 @default.
• W2157328736 hasConcept C14356644 @default.
• W2157328736 hasConcept C153911025 @default.
• W2157328736 hasConcept C159654299 @default.
• W2157328736 hasConcept C169760540 @default.
• W2157328736 hasConcept C171279029 @default.
• W2157328736 hasConcept C179369057 @default.
• W2157328736 hasConcept C182629451 @default.
• W2157328736 hasConcept C189413060 @default.
• W2157328736 hasConcept C189446657 @default.
• W2157328736 hasConcept C203014093 @default.
• W2157328736 hasConcept C203473731 @default.
• W2157328736 hasConcept C203880312 @default.
• W2157328736 hasConcept C2776694393 @default.
• W2157328736 hasConcept C2777093970 @default.
• W2157328736 hasConcept C2778024200 @default.
• W2157328736 hasConcept C2780758401 @default.
• W2157328736 hasConcept C86803240 @default.
• W2157328736 hasConcept C95444343 @default.
• W2157328736 hasConceptScore W2157328736C111684460 @default.
• W2157328736 hasConceptScore W2157328736C136015124 @default.

• next