FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2157460445> ?p ?o ?g. }

• W2157460445 abstract "Abstract Background It has become evident that host cells react to recombinant protein production with a variety of metabolic and intrinsic stresses such as the unfolded protein response (UPR) pathway. Additionally, environmental conditions such as growth temperature may have a strong impact on cell physiology and specific productivity. However, there is little information about the molecular reactions of the host cells on a genomic level, especially in context to recombinant protein secretion. For the first time, we monitored transcriptional regulation of a subset of marker genes in the common production host Pichia pastoris to gain insights into the general physiological status of the cells under protein production conditions, with the main focus on secretion stress related genes. Results Overexpression of the UPR activating transcription factor Hac1p was employed to identify UPR target genes in P. pastoris and the responses were compared to those known for Saccharomyces cerevisiae . Most of the folding/secretion related genes showed similar regulation patterns in both yeasts, whereas genes associated with the general stress response were differentially regulated. Secretion of an antibody Fab fragment led to induction of UPR target genes in P. pastoris , however not to the same magnitude as Hac1p overproduction. Overexpression of S. cerevisiae protein disulfide isomerase ( PDI1 ) enhances Fab secretion rates 1.9 fold, but did not relief UPR stress. Reduction of cultivation temperature from 25°C to 20°C led to a 1.4-fold increase of specific product secretion rate in chemostat cultivations, although the transcriptional levels of the product genes (Fab light and heavy chain) were significantly reduced at the lower temperature. A subset of folding related genes appeared to be down-regulated at the reduced temperature, whereas transcription of components of the ER associated degradation and the secretory transport was enhanced. Conclusion Monitoring of genomic regulation of marker genes with the transcriptional profiling method TRAC in P. pastoris revealed similarities and discrepancies of the responses compared to S. cerevisiae . Thus our results emphasize the importance to analyse the individual hosts under real production conditions instead of drawing conclusions from model organisms. Cultivation temperature has a significant influence on specific productivity that cannot be related just to thermodynamic effects, but strongly impacts the regulation of specific genes." @default.
• W2157460445 created "2016-06-24" @default.
• W2157460445 creator A5000974704 @default.
• W2157460445 creator A5009032164 @default.
• W2157460445 creator A5021155987 @default.
• W2157460445 creator A5021628047 @default.
• W2157460445 creator A5043243404 @default.
• W2157460445 creator A5050115274 @default.
• W2157460445 creator A5062107849 @default.
• W2157460445 creator A5074129234 @default.
• W2157460445 date "2007-06-19" @default.
• W2157460445 modified "2023-10-10" @default.
• W2157460445 title "Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions" @default.
• W2157460445 cites W1536737268 @default.
• W2157460445 cites W1544230703 @default.
• W2157460445 cites W1963580111 @default.
• W2157460445 cites W1970228759 @default.
• W2157460445 cites W1977498240 @default.
• W2157460445 cites W1978191146 @default.
• W2157460445 cites W1993212441 @default.
• W2157460445 cites W1994975348 @default.
• W2157460445 cites W1995801067 @default.
• W2157460445 cites W2005619516 @default.
• W2157460445 cites W2017562400 @default.
• W2157460445 cites W2018850937 @default.
• W2157460445 cites W2024583345 @default.
• W2157460445 cites W2026559943 @default.
• W2157460445 cites W2028875171 @default.
• W2157460445 cites W2029054117 @default.
• W2157460445 cites W2032400590 @default.
• W2157460445 cites W2039876512 @default.
• W2157460445 cites W2049656093 @default.
• W2157460445 cites W2051496342 @default.
• W2157460445 cites W2053803615 @default.
• W2157460445 cites W2063563712 @default.
• W2157460445 cites W2077607729 @default.
• W2157460445 cites W2081905674 @default.
• W2157460445 cites W2082581989 @default.
• W2157460445 cites W2088228878 @default.
• W2157460445 cites W2097235791 @default.
• W2157460445 cites W2098665480 @default.
• W2157460445 cites W2099567270 @default.
• W2157460445 cites W2103123883 @default.
• W2157460445 cites W2111796914 @default.
• W2157460445 cites W2115137627 @default.
• W2157460445 cites W2118542368 @default.
• W2157460445 cites W2120357935 @default.
• W2157460445 cites W2121010527 @default.
• W2157460445 cites W2125347557 @default.
• W2157460445 cites W2127754721 @default.
• W2157460445 cites W2131632095 @default.
• W2157460445 cites W2133112227 @default.
• W2157460445 cites W2137683543 @default.
• W2157460445 cites W2137755719 @default.
• W2157460445 cites W2140292029 @default.
• W2157460445 cites W2147901684 @default.
• W2157460445 cites W2162797237 @default.
• W2157460445 cites W2162891889 @default.
• W2157460445 cites W2192080449 @default.
• W2157460445 doi "https://doi.org/10.1186/1471-2164-8-179" @default.
• W2157460445 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1919374" @default.
• W2157460445 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17578563" @default.
• W2157460445 hasPublicationYear "2007" @default.
• W2157460445 type Work @default.
• W2157460445 sameAs 2157460445 @default.
• W2157460445 citedByCount "112" @default.
• W2157460445 countsByYear W21574604452012 @default.
• W2157460445 countsByYear W21574604452013 @default.
• W2157460445 countsByYear W21574604452014 @default.
• W2157460445 countsByYear W21574604452015 @default.
• W2157460445 countsByYear W21574604452016 @default.
• W2157460445 countsByYear W21574604452017 @default.
• W2157460445 countsByYear W21574604452018 @default.
• W2157460445 countsByYear W21574604452019 @default.
• W2157460445 countsByYear W21574604452020 @default.
• W2157460445 countsByYear W21574604452021 @default.
• W2157460445 countsByYear W21574604452022 @default.
• W2157460445 countsByYear W21574604452023 @default.
• W2157460445 crossrefType "journal-article" @default.
• W2157460445 hasAuthorship W2157460445A5000974704 @default.
• W2157460445 hasAuthorship W2157460445A5009032164 @default.
• W2157460445 hasAuthorship W2157460445A5021155987 @default.
• W2157460445 hasAuthorship W2157460445A5021628047 @default.
• W2157460445 hasAuthorship W2157460445A5043243404 @default.
• W2157460445 hasAuthorship W2157460445A5050115274 @default.
• W2157460445 hasAuthorship W2157460445A5062107849 @default.
• W2157460445 hasAuthorship W2157460445A5074129234 @default.
• W2157460445 hasBestOaLocation W21574604451 @default.
• W2157460445 hasConcept C104317684 @default.
• W2157460445 hasConcept C125111812 @default.
• W2157460445 hasConcept C139447449 @default.
• W2157460445 hasConcept C151730666 @default.
• W2157460445 hasConcept C2777576037 @default.
• W2157460445 hasConcept C2781372317 @default.
• W2157460445 hasConcept C2781452922 @default.
• W2157460445 hasConcept C3675279 @default.
• W2157460445 hasConcept C40767141 @default.
• W2157460445 hasConcept C49039625 @default.
• W2157460445 hasConcept C4916135 @default.
• W2157460445 hasConcept C523546767 @default.

• next