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• W2157460632 abstract "Six different compounds capable of mediating laccase-catalysed reactions have been tested by cyclic voltammetry. They exhibited quasi-reversible electrodic behaviour with formal redox potentials ranging from 150 to 800 mV (E(0)' vs. SCE). The immersion of a laccase-coated glassy carbon electrode (GCE) in mediator solutions generated large cathodic catalytic currents easily recorded by cyclic voltammetry at low-potential scan rates. This current showed two well-defined pH profiles, which correlated with the variation of the mediator redox potentials at the pH range tested. The relevant effect of temperature on the activity of laccase has been assessed here. Likewise, it was shown that the current record varied with the substrate concentration. This trend fitted Michaelis-Menten kinetics, which allowed us to give an estimation of the affinity of the fungal laccase for the different mediators." @default.
• W2157460632 created "2016-06-24" @default.
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• W2157460632 date "2002-12-01" @default.
• W2157460632 modified "2023-10-18" @default.
• W2157460632 title "Voltammetric monitoring of laccase-catalysed mediated reactions" @default.
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• W2157460632 doi "https://doi.org/10.1016/s1567-5394(02)00119-6" @default.
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