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• W2157521126 startingPage "2463" @default.
• W2157521126 abstract "We have used RNase H to study both the rates of ollgonucleotide hybridisation and dissociation at nearphysiological conditions. We have studied the Effects of oligonucleotide length, mismatch, and chemical modifications on oligonucleotide association and dissociation with RNA. Dissociation results were compared with standard thermal melting curves to compare relative stabilities evaluated by the two techniques. Although generally the two techniques correlate for the compounds evaluated, we found several instances where the thermal melting curves failed to reflect the relative stability of different oligonucleotldes at 37°C using near-physiological conditions. This study suggests that direct measurement of hybridization and dissociation of an ollgomer with RNA more accurately assesses the complicated kinetic scheme at 37°C using nearphyslologlcal conditions than thermal melting curves would predict." @default.
• W2157521126 created "2016-06-24" @default.
• W2157521126 creator A5023266063 @default.
• W2157521126 creator A5062960679 @default.
• W2157521126 date "1991-01-01" @default.
• W2157521126 modified "2023-09-23" @default.
• W2157521126 title "Hybridization and dissociation rates of phosphodiester or modified oligodeoxynucleotides with RNA at nearphysiological conditions." @default.
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• W2157521126 doi "https://doi.org/10.1093/nar/19.9.2463" @default.
• W2157521126 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/329458" @default.
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