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• W2157545351 startingPage "2604" @default.
• W2157545351 abstract "Replication-competent avian retroviruses, capable of transducing and expressing up to 2 kb of nonviral sequences, are now available to effect widespread gene transfer in chicken (chick) embryos (S. H. Hughes, J. J. Greenhouse, C. J. Petropoulos, and P. Sutrave, J. Virol. 61:3004-3012, 1987). We have constructed novel avian retroviral vectors that encode human placental alkaline phosphatase as a marker whose expression can be histochemically monitored. These vectors have been tested for expression by introducing them into the embryonic chick nervous system. They have revealed that the expression of retrovirally transduced genes can be spatially and temporally limited without the need for tissue-specific promoters. By varying the site and time of infection, targeted gene transfer can be confined to selected populations of neural cells over the course of several days, a time window that is sufficient for many key developmental processes. The capability of differentially infecting specific target populations may avoid confounding variables such as detrimental effects of a transduced gene on processes unrelated to the cells or tissue of interest. These vectors and methods thus should be useful in studies of the effect of transduced genes on the development of various organs and tissues during avian embryogenesis. In addition, the vectors will facilitate studies aimed at an understanding of viral infection and expression patterns." @default.
• W2157545351 created "2016-06-24" @default.
• W2157545351 creator A5032594307 @default.
• W2157545351 creator A5055156340 @default.
• W2157545351 date "1993-04-01" @default.
• W2157545351 modified "2023-10-11" @default.
• W2157545351 title "Replication-competent retroviral vectors encoding alkaline phosphatase reveal spatial restriction of viral gene expression/transduction in the chick embryo." @default.
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• W2157545351 doi "https://doi.org/10.1128/mcb.13.4.2604" @default.
• W2157545351 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/359596" @default.
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