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• W2157614078 abstract "Meiotic chromosome pairing was studied in introgression lines of cultivated tomato, Lycopersicon esculentum (= Solanum lycopersicum ), containing 1 or 2 chromosome segments from the wild species Solanum lycopersicoides . Genomic in situ hybridization (GISH) was used to compare the relative lengths at diakinesis of the different introgressed segments and to measure the chiasmate arm frequency for the chromosome pair involved in the introgression(s). Longer segments generally produced stronger GISH signals than shorter segments. GISH signal intensity also depended on whether or not an introgressed segment encompassed the centromeric region. For example, a 29 cM segment that included the centromeric region produced a stronger GISH signal than a 42 cM segment that did not. In each line the chromosome arm containing the homeologous segment showed a reduction in chiasmate arm frequency that was most pronounced in lines with long segments. This reduction was accompanied by an increased chiasmate arm frequency on the other arm. Double introgression lines, heterozygous in repulsion phase for 2 introgressions on opposite chromosome arms, showed a lower frequency of chiasmata than single introgression lines. Pairing failure, indicated by the presence of univalents, was highest in the double introgression and whole chromosome substitution lines. These results are discussed with respect to observations of suppressed recombination in these stocks and potential practical implications for reducing linkage drag in breeding programs." @default.
• W2157614078 created "2016-06-24" @default.
• W2157614078 creator A5072558446 @default.
• W2157614078 creator A5077396974 @default.
• W2157614078 date "2007-09-01" @default.
• W2157614078 modified "2023-09-26" @default.
• W2157614078 title "GISH analysis of meiotic chromosome pairing in<i>Solanum lycopersicoides</i>introgression lines of cultivated tomato" @default.
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• W2157614078 doi "https://doi.org/10.1139/g07-069" @default.
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