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- W2157622889 abstract "5-hydroxymethylcytosine (5hmC) has been suggested to be involved in various nucleic acid transactions and cellular processes, including transcriptional regulation, demethylation of 5-methylcytosine and stem cell pluripotency. We have identified an activity that preferentially catalyzes the cleavage of double-stranded 5hmC-modified DNA. Using biochemical methods we purified this activity from mouse liver extracts and demonstrate that the enzyme responsible for the cleavage of 5hmC-modified DNA is Endonuclease G (EndoG). We show that recombinant EndoG preferentially recognizes and cleaves a core sequence when one specific cytosine within that core sequence is hydroxymethylated. Additionally, we provide in vivo evidence that EndoG catalyzes the formation of double-stranded DNA breaks and that this cleavage is dependent upon the core sequence, EndoG and 5hmC. Finally, we demonstrate that the 5hmC modification can promote conservative recombination in an EndoG-dependent manner." @default.
- W2157622889 created "2016-06-24" @default.
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- W2157622889 creator A5020721787 @default.
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- W2157622889 date "2014-10-29" @default.
- W2157622889 modified "2023-10-13" @default.
- W2157622889 title "Endonuclease G preferentially cleaves 5-hydroxymethylcytosine-modified DNA creating a substrate for recombination" @default.
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- W2157622889 doi "https://doi.org/10.1093/nar/gku1032" @default.
- W2157622889 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4245937" @default.
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