FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2157627838> ?p ?o ?g. }

• W2157627838 endingPage "1565" @default.
• W2157627838 startingPage "1550" @default.
• W2157627838 abstract "The precise temporal-spatial regulation of the p21-activated serine-threonine kinase PAK at the plasma membrane is required for proper cytoskeletal reorganization and cell motility. However, the mechanism by which PAK localizes to focal adhesions has not yet been elucidated. Indirect binding of PAK to the focal adhesion protein paxillin via the Arf-GAP protein paxillin kinase linker (PKL) and PIX/Cool suggested a mechanism. In this report, we demonstrate an essential role for a paxillin–PKL interaction in the recruitment of activated PAK to focal adhesions. Similar to PAK, expression of activated Cdc42 and Rac1, but not RhoA, stimulated the translocation of PKL from a generally diffuse localization to focal adhesions. Expression of the PAK regulatory domain (PAK1–329) or the autoinhibitory domain (AID 83–149) induced PKL, PIX, and PAK localization to focal adhesions, indicating a role for PAK scaffold activation. We show PIX, but not NCK, binding to PAK is necessary for efficient focal adhesion localization of PAK and PKL, consistent with a PAK–PIX–PKL linkage. Although PAK activation is required, it is not sufficient for localization. The PKL amino terminus, containing the PIX-binding site, but lacking paxillin-binding subdomain 2 (PBS2), was unable to localize to focal adhesions and also abrogated PAK localization. An identical result was obtained after PKLΔPBS2 expression. Finally, neither PAK nor PKL was capable of localizing to focal adhesions in cells overexpressing paxillinΔLD4, confirming a requirement for this motif in recruitment of the PAK–PIX–PKL complex to focal adhesions. These results suggest a GTP-Cdc42/GTP-Rac triggered multistep activation cascade leading to the stimulation of the adaptor function of PAK, which through interaction with PIX provokes a functional PKL PBS2–paxillin LD4 association and consequent recruitment to focal adhesions. This mechanism is probably critical for the correct subcellular positioning of PAK, thereby influencing the ability of PAK to coordinate cytoskeletal reorganization associated with changes in cell shape and motility." @default.
• W2157627838 created "2016-06-24" @default.
• W2157627838 creator A5022094836 @default.
• W2157627838 creator A5046123085 @default.
• W2157627838 creator A5077106614 @default.
• W2157627838 date "2002-05-01" @default.
• W2157627838 modified "2023-10-16" @default.
• W2157627838 title "Paxillin-dependent Paxillin Kinase Linker and p21-Activated Kinase Localization to Focal Adhesions Involves a Multistep Activation Pathway" @default.
• W2157627838 cites W1488252042 @default.
• W2157627838 cites W1506345236 @default.
• W2157627838 cites W1662447390 @default.
• W2157627838 cites W1826215572 @default.
• W2157627838 cites W1938984736 @default.
• W2157627838 cites W1964024918 @default.
• W2157627838 cites W1967525449 @default.
• W2157627838 cites W1968826282 @default.
• W2157627838 cites W1975981720 @default.
• W2157627838 cites W1978985265 @default.
• W2157627838 cites W1982916034 @default.
• W2157627838 cites W1984967137 @default.
• W2157627838 cites W1986779411 @default.
• W2157627838 cites W1989987282 @default.
• W2157627838 cites W1990580084 @default.
• W2157627838 cites W1994962805 @default.
• W2157627838 cites W2000983089 @default.
• W2157627838 cites W2009023199 @default.
• W2157627838 cites W2009461629 @default.
• W2157627838 cites W2009575166 @default.
• W2157627838 cites W2014431633 @default.
• W2157627838 cites W2021289800 @default.
• W2157627838 cites W2022541364 @default.
• W2157627838 cites W2026469331 @default.
• W2157627838 cites W2027810531 @default.
• W2157627838 cites W2029693715 @default.
• W2157627838 cites W2038333370 @default.
• W2157627838 cites W2054779757 @default.
• W2157627838 cites W2061708016 @default.
• W2157627838 cites W2061987693 @default.
• W2157627838 cites W2062803349 @default.
• W2157627838 cites W2070190928 @default.
• W2157627838 cites W2077792958 @default.
• W2157627838 cites W2082365601 @default.
• W2157627838 cites W2086289346 @default.
• W2157627838 cites W2086405676 @default.
• W2157627838 cites W2087919267 @default.
• W2157627838 cites W2096791952 @default.
• W2157627838 cites W2099346800 @default.
• W2157627838 cites W2101480539 @default.
• W2157627838 cites W2102014126 @default.
• W2157627838 cites W2102787965 @default.
• W2157627838 cites W2105746559 @default.
• W2157627838 cites W2106140806 @default.
• W2157627838 cites W2109910885 @default.
• W2157627838 cites W2112652451 @default.
• W2157627838 cites W2112668385 @default.
• W2157627838 cites W2116312858 @default.
• W2157627838 cites W2116908725 @default.
• W2157627838 cites W2120337721 @default.
• W2157627838 cites W2121653060 @default.
• W2157627838 cites W2121810961 @default.
• W2157627838 cites W2122465481 @default.
• W2157627838 cites W2122875377 @default.
• W2157627838 cites W2123305987 @default.
• W2157627838 cites W2127441616 @default.
• W2157627838 cites W2128797396 @default.
• W2157627838 cites W2131206883 @default.
• W2157627838 cites W2134073082 @default.
• W2157627838 cites W2136316836 @default.
• W2157627838 cites W2137604636 @default.
• W2157627838 cites W2137618759 @default.
• W2157627838 cites W2144240811 @default.
• W2157627838 cites W2149150287 @default.
• W2157627838 cites W2149352954 @default.
• W2157627838 cites W2150172003 @default.
• W2157627838 cites W2150647809 @default.
• W2157627838 cites W2154524184 @default.
• W2157627838 cites W2155258698 @default.
• W2157627838 cites W2157647165 @default.
• W2157627838 cites W2161340336 @default.
• W2157627838 cites W2161946035 @default.
• W2157627838 cites W2165525793 @default.
• W2157627838 cites W2166782826 @default.
• W2157627838 cites W2168918180 @default.
• W2157627838 cites W2170779320 @default.
• W2157627838 cites W2178627498 @default.
• W2157627838 cites W4213174658 @default.
• W2157627838 cites W4250102918 @default.
• W2157627838 cites W78867158 @default.
• W2157627838 doi "https://doi.org/10.1091/mbc.02-02-0015" @default.
• W2157627838 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/111126" @default.
• W2157627838 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12006652" @default.
• W2157627838 hasPublicationYear "2002" @default.
• W2157627838 type Work @default.
• W2157627838 sameAs 2157627838 @default.
• W2157627838 citedByCount "153" @default.
• W2157627838 countsByYear W21576278382012 @default.
• W2157627838 countsByYear W21576278382013 @default.
• W2157627838 countsByYear W21576278382014 @default.

• next