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- W2157670939 abstract "SUMMARY Three purified cell-wall glycoprotein antigens, a, b and c , sensitive to sodium periodate and resistant to pronase, were extracted from mycelium of Micropolyspora faeni with aqueous phenol or trichloracetic acid. Pronase-sensitive, sodium periodate-resistant protein antigens were extracted from mycelium with aqueous phenol. Immunoelectrophoresis was a critical method of assessing purity of preparation. Antibodies to glycoprotein antigens only occurred in clinically defined cases of farmer's lung disease, whereas antibodies to protein antigens also occurred in symptomless farmers. Precipitins to a occurred in all cases of farmer's lung disease, and this antigen was isolated and purified. Fractionation of pooled sera from cases of farmer's lung disease showed that precipitins were IgG globulins, whereas latex-agglutinating antibodies were IgG, IgA and especially IgM globulins. It is suggested that farmer's lung may involve a cytotoxic type II reaction, in which glycoproteins adsorbed to tissue cells react with IgG, IgA and IgM immunoglobulins in the presence of complement, causing cellular damage." @default.
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- W2157670939 date "1974-02-01" @default.
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- W2157670939 title "Antibody responses in patients with farmer's lung disease to antigens fromMicropolyspora faeni" @default.
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- W2157670939 doi "https://doi.org/10.1017/s002217240002324x" @default.
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