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• W2157714362 endingPage "1497" @default.
• W2157714362 startingPage "1488" @default.
• W2157714362 abstract "The termini of Saccharomyces cerevisiae chromosomes consist of tracts of C1-3A (one to three cytosine and one adenine residue) sequences of approximately 450 base pairs in length. To gain insights into trans-acting factors at telomeres, high-copy-number linear and circular plasmids containing tracts of C1-3A sequences were introduced into S. cerevisiae. We devised a novel system to distinguish by color colonies that maintained the vector at 1 to 5, 20 to 50, and 100 to 400 copies per cell and used it to change the amount of telomeric DNA sequences per cell. An increase in the number of C1-3A sequences caused an increase in the length of telomeric C1-3A repeats that was proportional to plasmid copy number. Our data suggest that telomere growth is inhibited by a limiting factor(s) that specifically recognizes C1-3A sequences and that this factor can be effectively competed for by long tracts of C1-3A sequences at telomeres or on circular plasmids. Telomeres without this factor are exposed to processes that serve to lengthen chromosome ends." @default.
• W2157714362 created "2016-06-24" @default.
• W2157714362 creator A5006524357 @default.
• W2157714362 creator A5011723131 @default.
• W2157714362 date "1989-04-01" @default.
• W2157714362 modified "2023-09-25" @default.
• W2157714362 title "Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation." @default.
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• W2157714362 doi "https://doi.org/10.1128/mcb.9.4.1488" @default.
• W2157714362 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/362565" @default.
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