Matches in SemOpenAlex for { <https://semopenalex.org/work/W2157744648> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2157744648 endingPage "598" @default.
- W2157744648 startingPage "596" @default.
- W2157744648 abstract "Pseudomonas putida strain PP3 produces two dehalogenases, DehI and DehII, which belong to the group I and II alpha-haloacid dehalogenases, respectively. Group I dehalogenases catalyse the removal of halides from D-haloalkanoic acids and in some cases also the L-enantiomers, both substituted at their chiral centres. Studies of members of this group have resulted in the proposal of general catalytic mechanisms, although no structural information is available in order to better characterize their function. This work presents the initial stages of the structural investigation of the group I alpha-haloacid dehalogenase DehI. The DehI gene was cloned into a pET15b vector with an N-terminal His tag and expressed in Escherichia coli Nova Blue strain. Purified protein was crystallized in 25% PEG 3350, 0.4 M lithium sulfate and 0.1 M bis-tris buffer pH 6.0. The crystals were primitive monoclinic (space group P2(1)), with unit-cell parameters a = 68.32, b = 111.86, c = 75.13 A, alpha = 90, beta = 93.7, gamma = 90 degrees , and a complete native data set was collected. Molecular replacement is not an option for structure determination, so further experimental phasing methods will be necessary." @default.
- W2157744648 created "2016-06-24" @default.
- W2157744648 creator A5044051249 @default.
- W2157744648 creator A5045788938 @default.
- W2157744648 creator A5048370461 @default.
- W2157744648 creator A5060801806 @default.
- W2157744648 date "2008-06-07" @default.
- W2157744648 modified "2023-09-27" @default.
- W2157744648 title "Purification, crystallization and preliminary crystallographic analysis of DehI, a group I α-haloacid dehalogenase fromPseudomonas putidastrain PP3" @default.
- W2157744648 cites W1650796444 @default.
- W2157744648 cites W1939912622 @default.
- W2157744648 cites W1955343009 @default.
- W2157744648 cites W1970682329 @default.
- W2157744648 cites W2012860845 @default.
- W2157744648 cites W2034321085 @default.
- W2157744648 cites W2044834625 @default.
- W2157744648 cites W2054268140 @default.
- W2157744648 cites W2082424989 @default.
- W2157744648 cites W2130406589 @default.
- W2157744648 cites W2166414585 @default.
- W2157744648 cites W2339512621 @default.
- W2157744648 doi "https://doi.org/10.1107/s1744309108008865" @default.
- W2157744648 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2443979" @default.
- W2157744648 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18607084" @default.
- W2157744648 hasPublicationYear "2008" @default.
- W2157744648 type Work @default.
- W2157744648 sameAs 2157744648 @default.
- W2157744648 citedByCount "2" @default.
- W2157744648 countsByYear W21577446482018 @default.
- W2157744648 crossrefType "journal-article" @default.
- W2157744648 hasAuthorship W2157744648A5044051249 @default.
- W2157744648 hasAuthorship W2157744648A5045788938 @default.
- W2157744648 hasAuthorship W2157744648A5048370461 @default.
- W2157744648 hasAuthorship W2157744648A5060801806 @default.
- W2157744648 hasBestOaLocation W21577446482 @default.
- W2157744648 hasConcept C104317684 @default.
- W2157744648 hasConcept C115624301 @default.
- W2157744648 hasConcept C178790620 @default.
- W2157744648 hasConcept C181199279 @default.
- W2157744648 hasConcept C185592680 @default.
- W2157744648 hasConcept C2777166331 @default.
- W2157744648 hasConcept C2777485300 @default.
- W2157744648 hasConcept C547475151 @default.
- W2157744648 hasConcept C55493867 @default.
- W2157744648 hasConcept C61276311 @default.
- W2157744648 hasConcept C71240020 @default.
- W2157744648 hasConcept C8010536 @default.
- W2157744648 hasConceptScore W2157744648C104317684 @default.
- W2157744648 hasConceptScore W2157744648C115624301 @default.
- W2157744648 hasConceptScore W2157744648C178790620 @default.
- W2157744648 hasConceptScore W2157744648C181199279 @default.
- W2157744648 hasConceptScore W2157744648C185592680 @default.
- W2157744648 hasConceptScore W2157744648C2777166331 @default.
- W2157744648 hasConceptScore W2157744648C2777485300 @default.
- W2157744648 hasConceptScore W2157744648C547475151 @default.
- W2157744648 hasConceptScore W2157744648C55493867 @default.
- W2157744648 hasConceptScore W2157744648C61276311 @default.
- W2157744648 hasConceptScore W2157744648C71240020 @default.
- W2157744648 hasConceptScore W2157744648C8010536 @default.
- W2157744648 hasIssue "7" @default.
- W2157744648 hasLocation W21577446481 @default.
- W2157744648 hasLocation W21577446482 @default.
- W2157744648 hasLocation W21577446483 @default.
- W2157744648 hasLocation W21577446484 @default.
- W2157744648 hasOpenAccess W2157744648 @default.
- W2157744648 hasPrimaryLocation W21577446481 @default.
- W2157744648 hasRelatedWork W143825056 @default.
- W2157744648 hasRelatedWork W1969352129 @default.
- W2157744648 hasRelatedWork W1979358532 @default.
- W2157744648 hasRelatedWork W2070301709 @default.
- W2157744648 hasRelatedWork W2081595343 @default.
- W2157744648 hasRelatedWork W2082823139 @default.
- W2157744648 hasRelatedWork W2093692263 @default.
- W2157744648 hasRelatedWork W2262641225 @default.
- W2157744648 hasRelatedWork W2315457165 @default.
- W2157744648 hasRelatedWork W2354690460 @default.
- W2157744648 hasVolume "64" @default.
- W2157744648 isParatext "false" @default.
- W2157744648 isRetracted "false" @default.
- W2157744648 magId "2157744648" @default.
- W2157744648 workType "article" @default.