FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2157813174> ?p ?o ?g. }

• W2157813174 endingPage "113" @default.
• W2157813174 startingPage "103" @default.
• W2157813174 abstract "Pseudomonas aeruginosa is the dominant pathogen causing chronic lung infections in patients with cystic fibrosis (CF). After an initial phase characterized by intermittent colonizations, a chronic infection is established upon conversion of P. aeruginosa from the non-mucoid to the mucoid, alginate-overproducing phenotype. During the chronic infection the isolation of both mucoid and non-mucoid isolates in CF sputum samples is very common. The purpose of the present study was to establish, by sequence analysis, the types of mutations present in the algTmucABD operon in a large number of mucoid and non-mucoid P. aeruginosa isolates from Scandinavian CF patients and in in vitro-derived non-mucoid revertants. Mucoid (83) and non-mucoid isolates (103) from 91 Scandinavian patients with chronic P. aeruginosa infection and 24 non-mucoid isolates from intermittently colonized CF patients were investigated. In addition, 88 spontaneous non-mucoid revertants obtained in vitro from nine mucoid CF isolates were also included in the study. Mutations in mucA were found in 92 % of the mucoid and in up to 70 % of the non-mucoid isolates from chronically infected patients, indicating that the majority of non-mucoid isolates are revertants. None of the non-mucoid isolates from intermittently colonized CF patients harboured mucA mutations. Although algT has been considered an important gene for secondary-site mutations responsible for reversion to non-mucoidy, only 30 % of the mucA-mutated non-mucoid CF isolates had mutations in algT. In contrast, 83 % of the in vitro-derived spontaneous non-mucoid revertants had mutations in algT, showing that in the CF lung there is a selection for non-mucoid revertants with secondary-site mutations in genes other than algT. In addition, we report, to our knowledge for the first time, loss-of-function mutations in the negative regulators mucB and mucD in CF clinical isolates. In some of the CF isolates these mutations are associated with moderate alginate production. In conclusion, most non-mucoid isolates from chronically infected CF patients are revertants and the mechanism of revertance is algT-independent in the CF lung." @default.
• W2157813174 created "2016-06-24" @default.
• W2157813174 creator A5003930251 @default.
• W2157813174 creator A5005051796 @default.
• W2157813174 creator A5009112406 @default.
• W2157813174 creator A5016115500 @default.
• W2157813174 creator A5086191926 @default.
• W2157813174 creator A5087568222 @default.
• W2157813174 date "2008-01-01" @default.
• W2157813174 modified "2023-10-18" @default.
• W2157813174 title "Investigation of the algT operon sequence in mucoid and non-mucoid Pseudomonas aeruginosa isolates from 115 Scandinavian patients with cystic fibrosis and in 88 in vitro non-mucoid revertants" @default.
• W2157813174 cites W1486622462 @default.
• W2157813174 cites W1509847467 @default.
• W2157813174 cites W1563473226 @default.
• W2157813174 cites W1587799736 @default.
• W2157813174 cites W1615357436 @default.
• W2157813174 cites W1641710824 @default.
• W2157813174 cites W1674719483 @default.
• W2157813174 cites W1680922195 @default.
• W2157813174 cites W1757304708 @default.
• W2157813174 cites W1801144749 @default.
• W2157813174 cites W1819488180 @default.
• W2157813174 cites W1846517546 @default.
• W2157813174 cites W1875327167 @default.
• W2157813174 cites W1885759691 @default.
• W2157813174 cites W1889683180 @default.
• W2157813174 cites W1894649842 @default.
• W2157813174 cites W1947636021 @default.
• W2157813174 cites W1953284759 @default.
• W2157813174 cites W1955612312 @default.
• W2157813174 cites W1967432655 @default.
• W2157813174 cites W1980004985 @default.
• W2157813174 cites W2000427412 @default.
• W2157813174 cites W2002545509 @default.
• W2157813174 cites W2003343094 @default.
• W2157813174 cites W2020643343 @default.
• W2157813174 cites W2052675415 @default.
• W2157813174 cites W2066703675 @default.
• W2157813174 cites W2068880248 @default.
• W2157813174 cites W2070094351 @default.
• W2157813174 cites W2079332775 @default.
• W2157813174 cites W2095033422 @default.
• W2157813174 cites W2097438562 @default.
• W2157813174 cites W2099209753 @default.
• W2157813174 cites W2107016911 @default.
• W2157813174 cites W2108461176 @default.
• W2157813174 cites W2127060683 @default.
• W2157813174 cites W2127974336 @default.
• W2157813174 cites W2131298480 @default.
• W2157813174 cites W2134475541 @default.
• W2157813174 cites W2140585713 @default.
• W2157813174 cites W2141894296 @default.
• W2157813174 cites W2143326957 @default.
• W2157813174 cites W2145123043 @default.
• W2157813174 cites W2145887242 @default.
• W2157813174 cites W2147389566 @default.
• W2157813174 cites W2153528763 @default.
• W2157813174 cites W2153851167 @default.
• W2157813174 cites W2159645284 @default.
• W2157813174 cites W2163416143 @default.
• W2157813174 cites W2165797239 @default.
• W2157813174 cites W2166017852 @default.
• W2157813174 cites W2169118870 @default.
• W2157813174 cites W2169314851 @default.
• W2157813174 cites W2169545551 @default.
• W2157813174 cites W2172025654 @default.
• W2157813174 cites W4235252773 @default.
• W2157813174 cites W4253969210 @default.
• W2157813174 cites W4298242614 @default.
• W2157813174 doi "https://doi.org/10.1099/mic.0.2007/010421-0" @default.
• W2157813174 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18174130" @default.
• W2157813174 hasPublicationYear "2008" @default.
• W2157813174 type Work @default.
• W2157813174 sameAs 2157813174 @default.
• W2157813174 citedByCount "78" @default.
• W2157813174 countsByYear W21578131742012 @default.
• W2157813174 countsByYear W21578131742013 @default.
• W2157813174 countsByYear W21578131742014 @default.
• W2157813174 countsByYear W21578131742015 @default.
• W2157813174 countsByYear W21578131742016 @default.
• W2157813174 countsByYear W21578131742017 @default.
• W2157813174 countsByYear W21578131742018 @default.
• W2157813174 countsByYear W21578131742019 @default.
• W2157813174 countsByYear W21578131742020 @default.
• W2157813174 countsByYear W21578131742021 @default.
• W2157813174 countsByYear W21578131742022 @default.
• W2157813174 countsByYear W21578131742023 @default.
• W2157813174 crossrefType "journal-article" @default.
• W2157813174 hasAuthorship W2157813174A5003930251 @default.
• W2157813174 hasAuthorship W2157813174A5005051796 @default.
• W2157813174 hasAuthorship W2157813174A5009112406 @default.
• W2157813174 hasAuthorship W2157813174A5016115500 @default.
• W2157813174 hasAuthorship W2157813174A5086191926 @default.
• W2157813174 hasAuthorship W2157813174A5087568222 @default.
• W2157813174 hasBestOaLocation W21578131741 @default.
• W2157813174 hasConcept C104317684 @default.
• W2157813174 hasConcept C142724271 @default.
• W2157813174 hasConcept C203075996 @default.

• next