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• W2157888673 abstract "Microarrays are potential analyzing tools for genomics and proteomics researches, which is in needed of suitable substrate for coating and also hybridization of biomolecules.In this research, a thin film of oxidized agarose was prepared on the glass slides which previously coated with poly-L-lysine (PLL). Some of the aldehyde groups of the activated agarose linked covalently to PLL amine groups; also bound to the amino groups of biomolecules. These linkages were fixed by UV irradiation. The prepared substrates were compared to only agarose-coated and PLL-coated slides.RESULTS on atomic force microscope (AFM) demonstrated that agarose provided three-dimensional surface which had higher loading and bindig capacity for biomolecules than PLL-coated surface which had two-dimensional surface. In addition, the signal-to-noise ratio in hybridization reactions performed on the agarose-PLL coated substrates increased two fold and four fold compared to agarose and PLL coated substrates, respectively.The agarose-PLL microarrays had the highest signal (2546) and lowest background signal (205) in hybridization, suggesting that the prepared slides are suitable in analyzing wide concentration range of analytes." @default.
• W2157888673 created "2016-06-24" @default.
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• W2157888673 date "2013-12-01" @default.
• W2157888673 modified "2023-09-23" @default.
• W2157888673 title "An efficient covalent coating on glass slides for preparation of optical oligonucleotide microarrays." @default.
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• W2157888673 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3933803" @default.
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