Matches in SemOpenAlex for { <https://semopenalex.org/work/W2157938250> ?p ?o ?g. }
- W2157938250 endingPage "348" @default.
- W2157938250 startingPage "340" @default.
- W2157938250 abstract "To evaluate the role of acrosin in human sperm penetration of the zona pellucida (ZP), sperm-oocyte interaction was studied after acrosin activity was blocked with soybean trypsin inhibitor (SBTI). Oocytes that had failed to fertilize because of sperm pathology in a clinical in vitro fertilization program were used to assess sperm binding to and penetration into the ZP. The acrosome reaction of sperm bound to the ZP was determined using fluorescein-labeled Pisum sativum agglutinin after sperm were removed from the ZP. Acrosin activity, determined by a gelatin substrate film method, was severely inhibited by 2 mg/ml SBTI. Sperm motility and movement characteristics, assessed by a Hamilton-Thorn motility analyzer, were unchanged after 6-h incubation with SBTI. Inhibition of acrosin activity did not affect the number of sperm bound to the ZP but completely blocked sperm penetration of the ZP after a 5-h incubation. SBTI did not influence the spontaneous acrosome loss of sperm in culture medium after 6-h and 20-h incubations, but the percentage of acrosome-reacted sperm bound to the ZP was significantly reduced. It was concluded that acrosin activity plays a key role in sperm-zona interaction in humans. Motile sperm are unable to penetrate the ZP when acrosin activity is inhibited. This might result from interference with a phase of the sperm-ZP binding reaction or with a lytic action of acrosin. Also, acrosin may be involved in the acrosome reaction induced by sperm binding to the ZP." @default.
- W2157938250 created "2016-06-24" @default.
- W2157938250 creator A5004016736 @default.
- W2157938250 creator A5040485416 @default.
- W2157938250 date "1993-02-01" @default.
- W2157938250 modified "2023-10-11" @default.
- W2157938250 title "Inhibition of Acrosin Activity with a Trypsin Inhibitor Blocks Human Sperm Penetration of the Zona Pellucida" @default.
- W2157938250 cites W1073446951 @default.
- W2157938250 cites W151685000 @default.
- W2157938250 cites W1517725313 @default.
- W2157938250 cites W1878952451 @default.
- W2157938250 cites W1964490520 @default.
- W2157938250 cites W1977297266 @default.
- W2157938250 cites W197854883 @default.
- W2157938250 cites W1984911575 @default.
- W2157938250 cites W1996634581 @default.
- W2157938250 cites W1998287541 @default.
- W2157938250 cites W2024736916 @default.
- W2157938250 cites W2036536446 @default.
- W2157938250 cites W2044297412 @default.
- W2157938250 cites W2065634493 @default.
- W2157938250 cites W2067317165 @default.
- W2157938250 cites W2069462900 @default.
- W2157938250 cites W2077650907 @default.
- W2157938250 cites W2086353587 @default.
- W2157938250 cites W2097940344 @default.
- W2157938250 cites W2122057189 @default.
- W2157938250 cites W2122446224 @default.
- W2157938250 cites W2128456810 @default.
- W2157938250 cites W2210776648 @default.
- W2157938250 cites W2241005301 @default.
- W2157938250 cites W2244193310 @default.
- W2157938250 cites W2246851274 @default.
- W2157938250 cites W2247047461 @default.
- W2157938250 cites W2280128918 @default.
- W2157938250 cites W2346218172 @default.
- W2157938250 cites W2395122390 @default.
- W2157938250 cites W239957434 @default.
- W2157938250 cites W2408787377 @default.
- W2157938250 cites W2424756417 @default.
- W2157938250 cites W285583077 @default.
- W2157938250 cites W2907700089 @default.
- W2157938250 cites W560552711 @default.
- W2157938250 cites W594800272 @default.
- W2157938250 cites W626134520 @default.
- W2157938250 doi "https://doi.org/10.1095/biolreprod48.2.340" @default.
- W2157938250 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8439623" @default.
- W2157938250 hasPublicationYear "1993" @default.
- W2157938250 type Work @default.
- W2157938250 sameAs 2157938250 @default.
- W2157938250 citedByCount "78" @default.
- W2157938250 countsByYear W21579382502012 @default.
- W2157938250 countsByYear W21579382502013 @default.
- W2157938250 countsByYear W21579382502017 @default.
- W2157938250 countsByYear W21579382502018 @default.
- W2157938250 countsByYear W21579382502019 @default.
- W2157938250 countsByYear W21579382502020 @default.
- W2157938250 countsByYear W21579382502021 @default.
- W2157938250 countsByYear W21579382502022 @default.
- W2157938250 countsByYear W21579382502023 @default.
- W2157938250 crossrefType "journal-article" @default.
- W2157938250 hasAuthorship W2157938250A5004016736 @default.
- W2157938250 hasAuthorship W2157938250A5040485416 @default.
- W2157938250 hasBestOaLocation W21579382501 @default.
- W2157938250 hasConcept C105702510 @default.
- W2157938250 hasConcept C16685009 @default.
- W2157938250 hasConcept C196843134 @default.
- W2157938250 hasConcept C2776010925 @default.
- W2157938250 hasConcept C2776690073 @default.
- W2157938250 hasConcept C2777447553 @default.
- W2157938250 hasConcept C2777632594 @default.
- W2157938250 hasConcept C2778252442 @default.
- W2157938250 hasConcept C2779995066 @default.
- W2157938250 hasConcept C2781087480 @default.
- W2157938250 hasConcept C71924100 @default.
- W2157938250 hasConcept C86803240 @default.
- W2157938250 hasConcept C88972607 @default.
- W2157938250 hasConcept C95444343 @default.
- W2157938250 hasConceptScore W2157938250C105702510 @default.
- W2157938250 hasConceptScore W2157938250C16685009 @default.
- W2157938250 hasConceptScore W2157938250C196843134 @default.
- W2157938250 hasConceptScore W2157938250C2776010925 @default.
- W2157938250 hasConceptScore W2157938250C2776690073 @default.
- W2157938250 hasConceptScore W2157938250C2777447553 @default.
- W2157938250 hasConceptScore W2157938250C2777632594 @default.
- W2157938250 hasConceptScore W2157938250C2778252442 @default.
- W2157938250 hasConceptScore W2157938250C2779995066 @default.
- W2157938250 hasConceptScore W2157938250C2781087480 @default.
- W2157938250 hasConceptScore W2157938250C71924100 @default.
- W2157938250 hasConceptScore W2157938250C86803240 @default.
- W2157938250 hasConceptScore W2157938250C88972607 @default.
- W2157938250 hasConceptScore W2157938250C95444343 @default.
- W2157938250 hasIssue "2" @default.
- W2157938250 hasLocation W21579382501 @default.
- W2157938250 hasLocation W21579382502 @default.
- W2157938250 hasOpenAccess W2157938250 @default.
- W2157938250 hasPrimaryLocation W21579382501 @default.
- W2157938250 hasRelatedWork W1974623564 @default.