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• W2157977881 abstract "12 L INTRODUCTION 14 Pain defined 14 Nociception and pain transmission pathways 14 Neuropathic pain, a closer look 17 Current drug therapies for the treatment of neuropathic pain 18 Animal models of neuropathic pain spinal nerve ligation 20 Ectopic discharge 21 The rostral ventromedial medulla 24 A. On and off cells 25 B. Supraspinal modulation and descending facilitation of neuropathic pain. 28 C. Cholecystokinin as a mediator of descending facilitation from the RVM 30 Upregulation of dynorphin as a consequence of descending facilitation 32 Use of a targeted toxin to destroy |i opioid receptor-expressing cells 34 Summary of research goals 35 IL MATERIALS AND GENERAL METHODS 37 Animals used 37 RVM drug microinjection 37 Intrathecal drug administration 38 Spinal nerve injury 38 Spinal dorsolateral funiculus lesions 39 Radioligand binding 39 Tissue preparation 40 In situ hybridization of opioid receptor mRNA using cDNA probes 41 Subcloning and in vitro transcription of riboprobes 42 In situ hybridization of opioid |a, receptor and CCK2 receptor mRNA using riboprobes 43 Dynorphin immunoassay 45 CGRP release assay 45 Radioummunoassay for CGRP in superfusate 46 Immunostaining for the opioid |i receptor 46 Behavioral assessments 47" @default.
• W2157977881 created "2016-06-24" @default.
• W2157977881 creator A5057573688 @default.
• W2157977881 date "2003-01-01" @default.
• W2157977881 modified "2023-09-27" @default.
• W2157977881 title "THE CONTRIBUTION OF DESCENDING PAIN FACILITATION TO THE MAINTENANCE OF NEUROPATHIC PAIN" @default.
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