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• W2157987743 abstract "A method for the determination of salicylamide at concentrations between 25 and 350 ng ml−1 by use of matrix isopotential synchronous fluorescence spectrometry (MISF) in combination with derivative techniques is proposed. The method allows the determination of compounds in samples with unknown background fluorescence without the need for tedious pre-separation. Synchronous scans are performed along a trajectory that connects points of identical intensity in a three-dimensional fluorescence spectrum. The unknown analytical signal of the serum is suppressed from the MISF spectrum, by calculating its first derivative at λexc=324 nm and λem=392 nm. In order to ensure maximum sensitivity and adequate selectivity, the experimental variables affecting the fluorescence intensity of the salicylamide band at λexc=328 nm and λem=418 nm were studied. Based on the results, the determination was performed in an aqueous medium at pH 12 that was adjusted with a sodium phosphate/hydrogen phosphate buffer. Calibration graphs were subjected to a comprehensive statistical analysis. The error propagation has been considered in order to calculate the detection limit by the criterium of Clayton." @default.
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• W2157987743 date "2002-03-04" @default.
• W2157987743 modified "2023-09-27" @default.
• W2157987743 title "Direct determination of salicylamide in serum by matrix isopotential synchronous fluorimetry" @default.
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• W2157987743 doi "https://doi.org/10.1016/s0039-9140(01)00574-4" @default.
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