Matches in SemOpenAlex for { <https://semopenalex.org/work/W2158046315> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2158046315 endingPage "341" @default.
- W2158046315 startingPage "336" @default.
- W2158046315 abstract "It has been shown recently that some analogues of clinically ineffective trans -diamminedichloroplatinum(II) (transplatin) exhibit antitumor activity. This finding has inverted the empirical structure-antitumor activity relationships delineated for platinum(II) complexes, according to which only the cis geometry of leaving ligands in the bifunctional platinum complexes is therapeutically active. As a result, interactions of trans platinum compounds with DNA, which is the main pharmacological target of platinum anticancer drugs, are of great interest. The present paper describes the DNA binding of antitumor trans -[PtCl 2 ( E -imino ether) 2 ] complex ( trans-EE ) in a cell-free medium, which has been investigated using three experimental approaches. They involve thiourea as a probe of monofunctional DNA adducts of platinum(II) complexes with two leaving ligands in the trans configuration, ethidium bromide as a probe for distinguishing between monofunctional and bifunctional DNA adducts of platinum complexes and HPLC analysis of the platinated DNA enzymatically digested to nucleosides. The results show that bifunctional trans-EE preferentially forms monofunctional adducts at guanine residues in double-helical DNA even when DNA is incubated with the platinum complex for a relatively long time (48 h at 37°C in 10 mM NaClO 4 ). It implies that antitumor trans-EE modifies DNA in a different way than clinically ineffective transplatin, which forms prevalent amount of bifunctional DNA adducts after 48 h. This result has been interpreted to mean that the major adduct of trans-EE , occurring in DNA even after long reaction times, is a monofunctional adduct in which the reactivity of the second leaving group is markedly reduced. It has been suggested that the different properties of the adducts formed on DNA by transplatin and trans-EE are relevant to their distinct clinical efficacy." @default.
- W2158046315 created "2016-06-24" @default.
- W2158046315 creator A5081885555 @default.
- W2158046315 date "1996-01-15" @default.
- W2158046315 modified "2023-10-13" @default.
- W2158046315 title "DNA adducts of antitumor trans-[PtCl2 (E-imino ether)2]" @default.
- W2158046315 cites W1851015666 @default.
- W2158046315 cites W1976453250 @default.
- W2158046315 cites W1976939267 @default.
- W2158046315 cites W1977218968 @default.
- W2158046315 cites W1978775814 @default.
- W2158046315 cites W1979406893 @default.
- W2158046315 cites W1982759189 @default.
- W2158046315 cites W1984665941 @default.
- W2158046315 cites W1987940280 @default.
- W2158046315 cites W1993384720 @default.
- W2158046315 cites W1994987544 @default.
- W2158046315 cites W1996564711 @default.
- W2158046315 cites W1996803803 @default.
- W2158046315 cites W2001652098 @default.
- W2158046315 cites W2031168662 @default.
- W2158046315 cites W2045388444 @default.
- W2158046315 cites W2055080715 @default.
- W2158046315 cites W2074398149 @default.
- W2158046315 cites W2103902036 @default.
- W2158046315 cites W2165822018 @default.
- W2158046315 doi "https://doi.org/10.1093/nar/24.2.336" @default.
- W2158046315 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/145631" @default.
- W2158046315 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8628659" @default.
- W2158046315 hasPublicationYear "1996" @default.
- W2158046315 type Work @default.
- W2158046315 sameAs 2158046315 @default.
- W2158046315 citedByCount "80" @default.
- W2158046315 countsByYear W21580463152012 @default.
- W2158046315 countsByYear W21580463152013 @default.
- W2158046315 countsByYear W21580463152014 @default.
- W2158046315 countsByYear W21580463152015 @default.
- W2158046315 countsByYear W21580463152016 @default.
- W2158046315 countsByYear W21580463152017 @default.
- W2158046315 countsByYear W21580463152022 @default.
- W2158046315 crossrefType "journal-article" @default.
- W2158046315 hasAuthorship W2158046315A5081885555 @default.
- W2158046315 hasBestOaLocation W21580463151 @default.
- W2158046315 hasConcept C104317684 @default.
- W2158046315 hasConcept C108204754 @default.
- W2158046315 hasConcept C161790260 @default.
- W2158046315 hasConcept C178790620 @default.
- W2158046315 hasConcept C185592680 @default.
- W2158046315 hasConcept C2776907736 @default.
- W2158046315 hasConcept C2778301229 @default.
- W2158046315 hasConcept C2780407432 @default.
- W2158046315 hasConcept C2780443040 @default.
- W2158046315 hasConcept C2780538656 @default.
- W2158046315 hasConcept C512185932 @default.
- W2158046315 hasConcept C518104683 @default.
- W2158046315 hasConcept C552990157 @default.
- W2158046315 hasConcept C55493867 @default.
- W2158046315 hasConcept C71240020 @default.
- W2158046315 hasConcept C86803240 @default.
- W2158046315 hasConceptScore W2158046315C104317684 @default.
- W2158046315 hasConceptScore W2158046315C108204754 @default.
- W2158046315 hasConceptScore W2158046315C161790260 @default.
- W2158046315 hasConceptScore W2158046315C178790620 @default.
- W2158046315 hasConceptScore W2158046315C185592680 @default.
- W2158046315 hasConceptScore W2158046315C2776907736 @default.
- W2158046315 hasConceptScore W2158046315C2778301229 @default.
- W2158046315 hasConceptScore W2158046315C2780407432 @default.
- W2158046315 hasConceptScore W2158046315C2780443040 @default.
- W2158046315 hasConceptScore W2158046315C2780538656 @default.
- W2158046315 hasConceptScore W2158046315C512185932 @default.
- W2158046315 hasConceptScore W2158046315C518104683 @default.
- W2158046315 hasConceptScore W2158046315C552990157 @default.
- W2158046315 hasConceptScore W2158046315C55493867 @default.
- W2158046315 hasConceptScore W2158046315C71240020 @default.
- W2158046315 hasConceptScore W2158046315C86803240 @default.
- W2158046315 hasIssue "2" @default.
- W2158046315 hasLocation W21580463151 @default.
- W2158046315 hasLocation W21580463152 @default.
- W2158046315 hasLocation W21580463153 @default.
- W2158046315 hasLocation W21580463154 @default.
- W2158046315 hasOpenAccess W2158046315 @default.
- W2158046315 hasPrimaryLocation W21580463151 @default.
- W2158046315 hasRelatedWork W2049382188 @default.
- W2158046315 hasRelatedWork W2050682106 @default.
- W2158046315 hasRelatedWork W2084713457 @default.
- W2158046315 hasRelatedWork W2099637298 @default.
- W2158046315 hasRelatedWork W2133125857 @default.
- W2158046315 hasRelatedWork W2139700768 @default.
- W2158046315 hasRelatedWork W2158046315 @default.
- W2158046315 hasRelatedWork W2470403698 @default.
- W2158046315 hasRelatedWork W3025989039 @default.
- W2158046315 hasRelatedWork W2741089712 @default.
- W2158046315 hasVolume "24" @default.
- W2158046315 isParatext "false" @default.
- W2158046315 isRetracted "false" @default.
- W2158046315 magId "2158046315" @default.
- W2158046315 workType "article" @default.