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• W2158052175 endingPage "13446" @default.
• W2158052175 startingPage "13436" @default.
• W2158052175 abstract "ABSTRACT Influenza pandemics occur when influenza A viruses (IAV) adapted to other host species enter humans and spread through the population. Pandemics are relatively rare due to host restriction of IAV: strains adapted to nonhuman species do not readily infect, replicate in, or transmit among humans. IAV can overcome host restriction through reassortment or adaptive evolution, and these are mechanisms by which pandemic strains arise in nature. To identify mutations that facilitate growth of avian IAV in humans, we have adapted influenza A/duck/Alberta/35/1976 (H1N1) (dk/AB/76) virus to a high-growth phenotype in differentiated human tracheo-bronchial epithelial (HTBE) cells. Following 10 serial passages of three independent lineages, the bulk populations showed similar growth in HTBE cells to that of a human seasonal virus. The coding changes present in six clonal isolates were determined. The majority of changes were located in the polymerase complex and nucleoprotein (NP), and all isolates carried mutations in the PB2 627 domain and regions of NP thought to interact with PB2. Using reverse genetics, the impact on growth and polymerase activity of individual and paired mutations in PB2 and NP was evaluated. The results indicate that coupling of the mammalian-adaptive mutation PB2 E627K or Q591K to selected mutations in NP further augments the growth of the corresponding viruses. In addition, minimal combinations of three (PB2 Q236H, E627K, and NP N309K) or two (PB2 Q591K and NP S50G) mutations were sufficient to recapitulate the efficient growth in HTBE cells of dk/AB/76 viruses isolated after 10 passages in this substrate. IMPORTANCE Influenza A viruses adapted to birds do not typically grow well in humans. However, as has been seen recently with H5N1 and H7N9 subtype viruses, productive and virulent infection of humans with avian influenza viruses can occur. The ability of avian influenza viruses to adapt to new host species is a consequence of their high mutation rate that supports their zoonotic potential. Understanding of the adaptation of avian viruses to mammals strengthens public health efforts aimed at controlling influenza. In particular, it is critical to know how readily and through mutation to which functional components avian influenza viruses gain the ability to grow efficiently in humans. Our data show that as few as three mutations, in the PB2 and NP proteins, support robust growth of a low-pathogenic, H1N1 duck isolate in primary human respiratory cells." @default.
• W2158052175 created "2016-06-24" @default.
• W2158052175 creator A5004889712 @default.
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• W2158052175 creator A5060750749 @default.
• W2158052175 creator A5078913579 @default.
• W2158052175 date "2014-11-15" @default.
• W2158052175 modified "2023-10-18" @default.
• W2158052175 title "Mutations to PB2 and NP Proteins of an Avian Influenza Virus Combine To Confer Efficient Growth in Primary Human Respiratory Cells" @default.
• W2158052175 cites W12201287 @default.
• W2158052175 cites W1486091801 @default.
• W2158052175 cites W1515053395 @default.
• W2158052175 cites W1533200296 @default.
• W2158052175 cites W1538492542 @default.
• W2158052175 cites W1544994312 @default.
• W2158052175 cites W1555885901 @default.
• W2158052175 cites W1603867145 @default.
• W2158052175 cites W1964702822 @default.
• W2158052175 cites W1971178088 @default.
• W2158052175 cites W1973122974 @default.
• W2158052175 cites W1973948544 @default.
• W2158052175 cites W1976054800 @default.
• W2158052175 cites W1989524330 @default.
• W2158052175 cites W1996311210 @default.
• W2158052175 cites W1996783000 @default.
• W2158052175 cites W1998938204 @default.
• W2158052175 cites W2006647256 @default.
• W2158052175 cites W2008442521 @default.
• W2158052175 cites W2010130893 @default.
• W2158052175 cites W2014169878 @default.
• W2158052175 cites W2018594231 @default.
• W2158052175 cites W2020316997 @default.
• W2158052175 cites W2021175627 @default.
• W2158052175 cites W2023823133 @default.
• W2158052175 cites W2024323589 @default.
• W2158052175 cites W2037770042 @default.
• W2158052175 cites W2039817228 @default.
• W2158052175 cites W2045934762 @default.
• W2158052175 cites W2046378278 @default.
• W2158052175 cites W2048010092 @default.
• W2158052175 cites W2049740179 @default.
• W2158052175 cites W2056732680 @default.
• W2158052175 cites W2057745517 @default.
• W2158052175 cites W2062364477 @default.
• W2158052175 cites W2062869638 @default.
• W2158052175 cites W2063295208 @default.
• W2158052175 cites W2063350987 @default.
• W2158052175 cites W2069358581 @default.
• W2158052175 cites W2069971894 @default.
• W2158052175 cites W2071412733 @default.
• W2158052175 cites W2071427566 @default.
• W2158052175 cites W2072237100 @default.
• W2158052175 cites W2072676693 @default.
• W2158052175 cites W2073494242 @default.
• W2158052175 cites W2092903823 @default.
• W2158052175 cites W2096507556 @default.
• W2158052175 cites W2097317268 @default.
• W2158052175 cites W2100985160 @default.
• W2158052175 cites W2101094055 @default.
• W2158052175 cites W2103773683 @default.
• W2158052175 cites W2103904572 @default.
• W2158052175 cites W2107328689 @default.
• W2158052175 cites W2107651145 @default.
• W2158052175 cites W2110480900 @default.
• W2158052175 cites W2111785766 @default.
• W2158052175 cites W2112170460 @default.
• W2158052175 cites W2112790267 @default.
• W2158052175 cites W2113223606 @default.
• W2158052175 cites W2113557726 @default.
• W2158052175 cites W2114289644 @default.
• W2158052175 cites W2118794822 @default.
• W2158052175 cites W2120113718 @default.
• W2158052175 cites W2121378139 @default.
• W2158052175 cites W2126927090 @default.
• W2158052175 cites W2128025490 @default.
• W2158052175 cites W2130654686 @default.
• W2158052175 cites W2131855107 @default.
• W2158052175 cites W2134489190 @default.
• W2158052175 cites W2135386122 @default.
• W2158052175 cites W2137378887 @default.
• W2158052175 cites W2144056263 @default.
• W2158052175 cites W2146389912 @default.
• W2158052175 cites W2147586301 @default.
• W2158052175 cites W2148429348 @default.
• W2158052175 cites W2148994226 @default.
• W2158052175 cites W2151174547 @default.
• W2158052175 cites W2154107622 @default.
• W2158052175 cites W2158112812 @default.
• W2158052175 cites W2160484484 @default.
• W2158052175 cites W2160881014 @default.
• W2158052175 cites W2168720667 @default.
• W2158052175 cites W2169886642 @default.
• W2158052175 cites W4296590891 @default.
• W2158052175 doi "https://doi.org/10.1128/jvi.01093-14" @default.

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