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- W2158133119 abstract "Phomopsis azadirachtae is the causal agent of destructive die-back disease of neem. The molecular mechanism of pathogenicity is not clear. Pectinases, cellulases, hemicellulases and ligninases have been extensively studied because of their plant cell wall degrading nature. Hence, this study describes the screening and assay of extracellular enzymes produced by the pathogen. The pathogenicity enzymes screened were: laccase, polygalacturonase, xylanase, amylase, cellulase, protease and lipase. A total of fifteen isolates of Phomopsis azadirachtae were from different agroclimatic regions of Karnataka, Tamil Nadu and Andhra Pradesh from diseased samples. The isolates were subjected to screening of the above mentioned enzymes on solid media supplemented with respective substrates. All the isolates were found to produce polygalacturonase, laccase, protease and xylanase. Polygalacturonase and xylanase were further assayed in order to quantify the enzyme produced in all the isolates. In addition to pathogenicity enzymes, isolates were also found to produce some industrially important enzymes such as L-asparaginase and urease." @default.
- W2158133119 created "2016-06-24" @default.
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- W2158133119 date "2013-01-01" @default.
- W2158133119 modified "2023-09-26" @default.
- W2158133119 title "Screening and assay of extracellular enzymes in Phomopsis azadirachtae causing die-back disease of neem." @default.
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