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- W2158263148 abstract "(Recibido:15 octubre, 2009; aceptado: 2 febrero, 2010) Summary The aim of this study was to establish the protocol of conventional and real time PCR for amplification of Mycobacterium avium subsp. paratuberculosis (MAP) genome from bovine fecal samples, as a way to define strategies for establishing a prevention and control program in a dairy herd at the Universidad de Antioquia (Medellin, Colombia). Fecal samples were individually taken of clinical healthy cows or cows with diarrhea bred in a herd enzootic for Johne’s disease, were processed them for culture in liquid Middlebrook 7H9 media supplemented with mycobactin under two different protocols: with or without inhibitors. Fecal samples from clinically healthy cows were used as negative control. Conventional and real time PCR were performed with MAP DNA obtained of fecal or cultured samples. The MAP- specific IS900 segment was amplified by using the respective forward and reverse primers. DNA isolated from a reference MAP strain was used as positive control of PCR. Data were analyzed by descriptive statistics and simple regression analysis between PCR and culture results were performed. All samples cultured in media with or without mycobactin gave a positive result compatible with MAP growth. However, only 13,3% of samples were positive by real time PCR. There was no relationship neither between PCR and" @default.
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- W2158263148 date "2010-03-01" @default.
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- W2158263148 title "Identificação de Mycobacterium avium subespécie paratuberculosis por PCR e definição do programa de controle da paratuberculose em rebanhos bovinos leiteiros" @default.
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