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• W2158285441 abstract "OBJECTIVE: Research on human embryos requires models to explore future techniques and to improve currents. Due to this interest, we aimed to determine whether TPN would be an acceptable model for in vitro development. Thus, we studied the cleavage pattern and developmental of TPN, depending on the fertilization technique used and compared TPN development record with sibling bipronucleated (BPN), correctly fertilized embryos. DESIGN: Observational and retrospective report approved by the Ethical Committee of UI-IVI, 14/2003 Spanish Law. MATERIALS AND METHODS: The day after insemination, TPN were identified and banking by vitrification. After obtaining informed and signed consent from parents, 37 TPN from ICSI (TPN-I) and 37 TPN from IVF (TPN-F) were warmed and cultured in 50μl HTF at 5% CO2 and 37°C for 3 days. Number of cells was daily registered. Then, embryos were put in 50μl CCM for 2 days and growth to morula (M) and blastocyst (B) checked. To determine the adequacy of TPN such as model, their in vitro development was retrospectively compared to 843 sibling BPN from IVF (BPN-F) and 805 from ICSI (BPN-I). RESULTS: On day 2, TPN-I cleaved like BPN (97%, in average); however, more TPN-F blocked on day 1 (10.8%; p<0.05), but had more cells than TPN-I and BPN controls as well (table). Out of cleaved embryos, 89% progressed to day 3, regardless ploidy or fertilization technique from they came. On day 3, TPN-I and TPN-F had fewer cells than controls, with the TPN-I having the fewest. The embryo ability to grow to M+B varied according to both ploidy and fertilization origin. Thus, more IVF-derived BPN grew to M+B than any othes, including ICSI controls. Concerning TPN, comparable percentages of M+B were obtained, regardless IVF or ICSI origin (in average, 43%). CONCLUSIONS: On early days of development, TPN-F had more cells, probably due to fastened initial cleavage or, increased number of cells due to an augmented multiplying factor at the fisrt cleavage. We thought that the double centriolar inheritance in dispermic TPN-F would be responsible. This is supported by the comparable cell counting found in both controls and TPN-I, since majority of TPN-I are digynic in origin and, like BPN, resulted from monospermic (unicentriolar) inheritance. Concerning extended in vitro culture, development to M-B was impaired in TPN, probably for ploidy and/or parental endowment. Thus, TPN from IVF or ICSI do not represent an ultimate model for studying the human development." @default.
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• W2158285441 date "2008-09-01" @default.
• W2158285441 modified "2023-09-27" @default.
• W2158285441 title "Do tripronucleated embryos (TPN) offer a reliable model of regular in vitro development?" @default.
• W2158285441 doi "https://doi.org/10.1016/j.fertnstert.2008.07.1589" @default.
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