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• W2158291260 endingPage "3709" @default.
• W2158291260 startingPage "3704" @default.
• W2158291260 abstract "The generation of high levels of new catalytic activities on natural and artificial protein scaffolds is a major goal of enzyme engineering. Here, we used random mutagenesis and selection in vivo to establish a sugar isomerisation reaction on both a natural (βα) 8 -barrel enzyme and a catalytically inert chimeric (βα) 8 -barrel scaffold, which was generated by the recombination of 2 (βα) 4 -half barrels. The best evolved variants show turnover numbers and substrate affinities that are similar to those of wild-type enzymes catalyzing the same reaction. The determination of the crystal structure of the most proficient variant allowed us to model the substrate sugar in the novel active site and to elucidate the mechanistic basis of the newly established activity. The results demonstrate that natural and inert artificial protein scaffolds can be converted into highly proficient enzymes in the laboratory, and provide insights into the mechanisms of enzyme evolution." @default.
• W2158291260 created "2016-06-24" @default.
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• W2158291260 date "2009-03-10" @default.
• W2158291260 modified "2023-09-23" @default.
• W2158291260 title "Establishing wild-type levels of catalytic activity on natural and artificial (βα) ₈ -barrel protein scaffolds" @default.
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• W2158291260 doi "https://doi.org/10.1073/pnas.0810342106" @default.
• W2158291260 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2656144" @default.
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