FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2158331582> ?p ?o ?g. }

• W2158331582 endingPage "5942" @default.
• W2158331582 startingPage "5936" @default.
• W2158331582 abstract "ABSTRACT Archaeal protein trafficking is a poorly characterized process. While putative type I signal peptidase genes have been identified in sequenced genomes for many archaea, no biochemical data have been presented to confirm that the gene product possesses signal peptidase activity. In this study, the putative type I signal peptidase gene in Methanococcus voltae was cloned and overexpressed in Escherichia coli , the membranes of which were used as the enzyme source in an in vitro peptidase assay. A truncated, His-tagged form of the M. voltae S-layer protein was generated for use as the substrate to monitor the signal peptidase activity. With M. voltae membranes as the enzyme source, signal peptidase activity in vitro was optimal between 30 and 40°C; it was dependent on a low concentration of KCl or NaCl but was effective over a broad concentration range up to 1 M. Processing of the M. voltae S-layer protein at the predicted cleavage site (confirmed by N-terminal sequencing) was demonstrated with the overexpressed archaeal gene product. Although E. coli signal peptidase was able to correctly process the signal peptide during overexpression of the M. voltae S-layer protein in vivo, the contribution of the E. coli signal peptidase to cleavage of the substrate in the in vitro assay was minimal since E. coli membranes alone did not show significant activity towards the S-layer substrate in in vitro assays. In addition, when the peptidase assays were performed in 1 M NaCl (a previously reported inhibitory condition for E. coli signal peptidase I), efficient processing of the substrate was observed only when the E. coli membranes contained overexpressed M. voltae signal peptidase. This is the first proof of expressed type I signal peptidase activity from a specific archaeal gene product." @default.
• W2158331582 created "2016-06-24" @default.
• W2158331582 creator A5066984826 @default.
• W2158331582 creator A5072539617 @default.
• W2158331582 date "2003-10-15" @default.
• W2158331582 modified "2023-10-14" @default.
• W2158331582 title "Cloning and Characterization of Archaeal Type I Signal Peptidase from Methanococcus voltae" @default.
• W2158331582 cites W1479888353 @default.
• W2158331582 cites W1500563017 @default.
• W2158331582 cites W1504149720 @default.
• W2158331582 cites W1565129749 @default.
• W2158331582 cites W1947962930 @default.
• W2158331582 cites W1953597614 @default.
• W2158331582 cites W1957311428 @default.
• W2158331582 cites W1968143836 @default.
• W2158331582 cites W1971120607 @default.
• W2158331582 cites W1995759416 @default.
• W2158331582 cites W2002422062 @default.
• W2158331582 cites W2003546739 @default.
• W2158331582 cites W2008683693 @default.
• W2158331582 cites W2038669207 @default.
• W2158331582 cites W2058145071 @default.
• W2158331582 cites W2061320085 @default.
• W2158331582 cites W2073269369 @default.
• W2158331582 cites W2074649033 @default.
• W2158331582 cites W2077091533 @default.
• W2158331582 cites W2094136809 @default.
• W2158331582 cites W2101108802 @default.
• W2158331582 cites W2115407181 @default.
• W2158331582 cites W2119452210 @default.
• W2158331582 cites W2134236489 @default.
• W2158331582 cites W2143288225 @default.
• W2158331582 cites W2152770371 @default.
• W2158331582 cites W2160888228 @default.
• W2158331582 cites W2166158613 @default.
• W2158331582 cites W263505340 @default.
• W2158331582 cites W4237138552 @default.
• W2158331582 cites W4239833500 @default.
• W2158331582 doi "https://doi.org/10.1128/jb.185.20.5936-5942.2003" @default.
• W2158331582 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/225044" @default.
• W2158331582 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14526003" @default.
• W2158331582 hasPublicationYear "2003" @default.
• W2158331582 type Work @default.
• W2158331582 sameAs 2158331582 @default.
• W2158331582 citedByCount "23" @default.
• W2158331582 countsByYear W21583315822013 @default.
• W2158331582 countsByYear W21583315822014 @default.
• W2158331582 countsByYear W21583315822015 @default.
• W2158331582 countsByYear W21583315822018 @default.
• W2158331582 countsByYear W21583315822019 @default.
• W2158331582 crossrefType "journal-article" @default.
• W2158331582 hasAuthorship W2158331582A5066984826 @default.
• W2158331582 hasAuthorship W2158331582A5072539617 @default.
• W2158331582 hasBestOaLocation W21583315822 @default.
• W2158331582 hasConcept C104317684 @default.
• W2158331582 hasConcept C10858879 @default.
• W2158331582 hasConcept C114883478 @default.
• W2158331582 hasConcept C150194340 @default.
• W2158331582 hasConcept C153911025 @default.
• W2158331582 hasConcept C167625842 @default.
• W2158331582 hasConcept C195286587 @default.
• W2158331582 hasConcept C2776878370 @default.
• W2158331582 hasConcept C2779190341 @default.
• W2158331582 hasConcept C547475151 @default.
• W2158331582 hasConcept C55493867 @default.
• W2158331582 hasConcept C86803240 @default.
• W2158331582 hasConceptScore W2158331582C104317684 @default.
• W2158331582 hasConceptScore W2158331582C10858879 @default.
• W2158331582 hasConceptScore W2158331582C114883478 @default.
• W2158331582 hasConceptScore W2158331582C150194340 @default.
• W2158331582 hasConceptScore W2158331582C153911025 @default.
• W2158331582 hasConceptScore W2158331582C167625842 @default.
• W2158331582 hasConceptScore W2158331582C195286587 @default.
• W2158331582 hasConceptScore W2158331582C2776878370 @default.
• W2158331582 hasConceptScore W2158331582C2779190341 @default.
• W2158331582 hasConceptScore W2158331582C547475151 @default.
• W2158331582 hasConceptScore W2158331582C55493867 @default.
• W2158331582 hasConceptScore W2158331582C86803240 @default.
• W2158331582 hasIssue "20" @default.
• W2158331582 hasLocation W21583315821 @default.
• W2158331582 hasLocation W21583315822 @default.
• W2158331582 hasLocation W21583315823 @default.
• W2158331582 hasOpenAccess W2158331582 @default.
• W2158331582 hasPrimaryLocation W21583315821 @default.
• W2158331582 hasRelatedWork W2040762413 @default.
• W2158331582 hasRelatedWork W2072604958 @default.
• W2158331582 hasRelatedWork W2074363799 @default.
• W2158331582 hasRelatedWork W2077091533 @default.
• W2158331582 hasRelatedWork W2099677026 @default.
• W2158331582 hasRelatedWork W2108799705 @default.
• W2158331582 hasRelatedWork W2120240328 @default.
• W2158331582 hasRelatedWork W2158331582 @default.
• W2158331582 hasRelatedWork W2400602584 @default.
• W2158331582 hasRelatedWork W2572680590 @default.
• W2158331582 hasVolume "185" @default.
• W2158331582 isParatext "false" @default.
• W2158331582 isRetracted "false" @default.
• W2158331582 magId "2158331582" @default.

• next