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- W2158333869 abstract "In vivo changes in the cytosolic pH of abscission zone (AZ) cells were visualized using confocal microscopic detection of the fluorescent pH-sensitive and intracellularly trapped dye, 2',7'-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF), driven by its acetoxymethyl ester. A specific and gradual increase in the cytosolic pH of AZ cells was observed during natural abscission of flower organs in Arabidopsis thaliana and wild rocket (Diplotaxis tenuifolia), and during flower pedicel abscission induced by flower removal in tomato (Solanum lycopersicum Mill). The alkalization pattern in the first two species paralleled the acceleration or inhibition of flower organ abscission induced by ethylene or its inhibitor 1-methylcyclopropene (1-MCP), respectively. Similarly, 1-MCP pre-treatment of tomato inflorescence explants abolished the pH increase in AZ cells and pedicel abscission induced by flower removal. Examination of the pH changes in the AZ cells of Arabidopsis mutants defective in both ethylene-induced (ctr1, ein2, eto4) and ethylene-independent (ida, nev7, dab5) abscission pathways confirmed these results. The data indicate that the pH changes in the AZ cells are part of both the ethylene-sensitive and -insensitive abscission pathways, and occur concomitantly with the execution of organ abscission. pH can affect enzymatic activities and/or act as a signal for gene expression. Changes in pH during abscission could occur via regulation of transporters in AZ cells, which might affect cytosolic pH. Indeed, four genes associated with pH regulation, vacuolar H(+)-ATPase, putative high-affinity nitrate transporter, and two GTP-binding proteins, were specifically up-regulated in tomato flower AZ following abscission induction, and 1-MCP reduced or abolished the increased expression." @default.
- W2158333869 created "2016-06-24" @default.
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- W2158333869 date "2014-12-10" @default.
- W2158333869 modified "2023-10-15" @default.
- W2158333869 title "Abscission of flowers and floral organs is closely associated with alkalization of the cytosol in abscission zone cells" @default.
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- W2158333869 cites W1963816148 @default.
- W2158333869 cites W1970939960 @default.
- W2158333869 cites W1974137133 @default.
- W2158333869 cites W2002533319 @default.
- W2158333869 cites W2004909915 @default.
- W2158333869 cites W2005496979 @default.
- W2158333869 cites W2009387459 @default.
- W2158333869 cites W2012103484 @default.
- W2158333869 cites W2013962341 @default.
- W2158333869 cites W2015065370 @default.
- W2158333869 cites W2016741878 @default.
- W2158333869 cites W2024343038 @default.
- W2158333869 cites W2030578655 @default.
- W2158333869 cites W2035212032 @default.
- W2158333869 cites W2035492137 @default.
- W2158333869 cites W2036182307 @default.
- W2158333869 cites W2043882244 @default.
- W2158333869 cites W2050307349 @default.
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- W2158333869 cites W2168092613 @default.
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- W2158333869 doi "https://doi.org/10.1093/jxb/eru483" @default.
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