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• W2158352972 endingPage "1382" @default.
• W2158352972 startingPage "1375" @default.
• W2158352972 abstract "Soluble HLA-DR401 or -DR404 tetramers containing a peptide corresponding to an immunodominant epitope from human GAD65 were used to analyze peripheral blood T-cells of newly diagnosed type 1 diabetic patients and at-risk subjects. Peripheral blood mononuclear cells were expanded on antigen-presenting cells presenting GAD65 peptide and subsequently activated with specific plate-bound class II-peptide monomers. T-cell activation defined in flow cytometry by CD4high and/or CD25 markers were observed in all type 1 diabetic patients and some at-risk subjects, but not in normal control subjects. The activated T-cells stained positive with tetramers containing the GAD65 epitope 555-567. Tetramer-positive cells were CD4high T-cells with high avidity for an immunodominant GAD65 T-cell epitope. Phenotyping of T-cells utilizing HLA class II tetramers provides a new tool to characterize the autoimmune response in type 1 diabetes." @default.
• W2158352972 created "2016-06-24" @default.
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• W2158352972 date "2002-05-01" @default.
• W2158352972 modified "2023-10-17" @default.
• W2158352972 title "Detection of GAD65-Specific T-Cells by Major Histocompatibility Complex Class II Tetramers in Type 1 Diabetic Patients and At-Risk Subjects" @default.
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• W2158352972 doi "https://doi.org/10.2337/diabetes.51.5.1375" @default.
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